4-Morpholineethanesulfonic acid Chemical Properties

Melting point:

>300 °C(lit.)

Boiling point:

102°C

Density 

1.208 (estimate)

refractive index 

1.5500 (estimate)

storage temp. 

2-8°C

solubility 

H₂O: 0.5 M at 20 °C, clear

form 

Powder

pka

pKa at 0.1M ionic: 0°, 6.38; 20°, 6.15; 37°, 5.98; pKa1 1.99; pKa2 6.21; pKa/°C -0.011

color 

White

PH

2.5-4.0 (25℃ in neat)

PH Range

5.5 - 6.7

Water Solubility 

185.2g/L at 20℃

λmax

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

Merck 

13,5929

BRN 

6350956

Stability:

Stable. Incompatible with strong oxidizing agents.

InChIKey

SXGZJKUKBWWHRA-UHFFFAOYSA-N

LogP

-2.2 at 20℃

CAS DataBase Reference

4432-31-9(CAS DataBase Reference)

EPA Substance Registry System

4-Morpholineethanesulfonic acid (4432-31-9)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-24/25-22

WGK Germany 

1

RTECS 

QE9104530

HS Code 

29349990

MSDS

• Language:English Provider:2-(N-Morpholino)ethanesulfonic acid
• Language:English Provider:SigmaAldrich

4-Morpholineethanesulfonic acid Usage And Synthesis

Application

4-Morpholineethanesulfonic acid (MES) is one of the good′s buffers, and the most extensively application is biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring and it does not form complexes with the majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes. MES has been also used as a component of the elution buffer used to elute plasma from α2-macroglobulin affinity column. It has been used as an activation buffer during antibody conjugation to microparticles and it has been used in the functionalization of microchannels during the creation of microfluidic-integrated surface plasmon resonance (SPR) platform for pathogen detection.

Chemical Properties

White/clear crystalline powder

Uses

MES solution has been used to activate microspheres. It has also been used as a component of activating solution to activate carboxyl groups of poly lactic-co-glycolic acid (PLGA).

Uses

Biological buffer.

Definition

ChEBI: 2-(N-morpholino)ethanesulfonic acid is a Good's buffer substance, pKa = 6.15 at 20 ℃. It is an organosulfonic acid and a MES. It is a conjugate acid of a 2-(N-morpholino)ethanesulfonate. It is a tautomer of a 2-(N-morpholiniumyl)ethanesulfonate.

General Description

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Flammability and Explosibility

Not classified

storage

Store at RT

Purification Methods

Crystallise MES from hot EtOH containing a little water. The picrate crystallises from EtOH and has m 178.8-182o. [Malkiel & Mason J Org Chem 8 199 1943, Beilstein 27 III/IV 370.]

4-Morpholineethanesulfonic acid(4432-31-9)Related Product Information

• Tridemorph
• Moroxydine
• Moroxydine hydrochloride
• Dimethomorph
• Ethanesulfonic acid
• Ascoric Acid
• MES monohydrate
• 2-methylthioadenosine-5’-o-diphosphate(2-mes-atp),sodiumsal
• HEPPS
• MES-K,MES POTASSIUM SALT,2-(N-Morpholino)ethanesulfonic acid potassium salt, 4-Morpholineethanesulfonic acid potassium salt
• 2-(N-Morpholino)ethanesulfonic acid hemisodium salt
• MES sodium salt
• 2-methylthioadenosine-5’-o-triphosphate(2-mes-atp),sodiumsal
• Methyl ethanesulfonate
• OVA IN MES
• MesBuffer
• 4-Morpholineethanesulfonic acid monohydrate, MES-d13,MES-d13,4-Morpholineethanesulfonic acid monohydrate, MES-d13
• 4-Morpholineethanesulfonic acid