5-(Ethylthio)-1H-tetrazole Chemical Properties

Melting point:

72-80 °C(lit.)

Boiling point:

81-82 °C

Density 

0.786 g/mL at 25 °C

Flash point:

42 °F

storage temp. 

2-8°C

solubility 

soluble, clear, colorless

form 

buffered aqueous solution

pka

3.89±0.10(Predicted)

color 

solid white

InChIKey

GONFBOIJNUKKST-UHFFFAOYSA-N

CAS DataBase Reference

89797-68-2(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi,Xn,F

Risk Statements 

36/37/38-44-36-20/21/22-11-5

Safety Statements 

26-35-36/37-16-36

RIDADR 

UN 1648 3/PG 2

WGK Germany 

3

Hazard Note 

Irritant

HazardClass 

4.1

PackingGroup 

III

HS Code 

29299000

MSDS

• Language:English Provider:SigmaAldrich

5-(Ethylthio)-1H-tetrazole Usage And Synthesis

Chemical Properties

White crystals

Application

5-(Ethylthio)-1H-tetrazole is used a powerful activator in DNA and RNA syntheses.

Preparation

5-(Ethylthio)-1H-Tetrazole is prepared from the corresponding alkyl thiocyanate and sodium azide through a [2?+?3] cycloaddition under phase transfer catalytic conditions (eq 1). The ratio of the solvent (water to toluene) as well as the reaction temperature are important factors in obtaining preparative yields of the alkylthiotetrazoles. 5-Ethylthio-1H-tetrazole has also been prepared using DMF as solvent albeit in much lower yield.

General Description

5-(Ethylthio)-1H-tetrazole (ETT) is an efficient activator, that activates nucleoside phosphoramidites towards condensation with a nucleoside to form dinucleoside phosphates during oligonucleotide synthesis.

Purification Methods

recrystallized from toluene.

5-(Ethylthio)-1H-tetrazole(89797-68-2)Related Product Information

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• ACTIVATOR REAGENT
• ACTIVATOR REAGENT
• LIPASE-PS ACTIVATOR REAGENT
• 5-(Ethylthio)-1H-tetrazole