7-AMINO-4-METHYL-COUMARIN-3-ACETIC ACID ESTER
7-AMINO-4-METHYL-COUMARIN-3-ACETIC ACID ESTER Basic information
- Product Name:
- 7-AMINO-4-METHYL-COUMARIN-3-ACETIC ACID ESTER
- Synonyms:
-
- 7-AMINO-4-METHYL-COUMARIN-3-ACETIC ACID ESTER
- AMCA-NHS ESTER
- MF:
- C12H11NO4
- MW:
- 233.22
- Mol File:
- Mol File
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7-AMINO-4-METHYL-COUMARIN-3-ACETIC ACID ESTER Chemical Properties
- solubility
- soluble in DMSO, DMF
- Appearance
- gray powder
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7-AMINO-4-METHYL-COUMARIN-3-ACETIC ACID ESTER Usage And Synthesis
Description
Activated NHS-ester of AMCA (aminomethyl coumarin acetate) dye. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.
AMCA is one of the brightest blue fluorescent dyes. This fluorophore has a relatively large Stoke’s shift, high resistance to photobleaching, and pH-independent fluorescence from pH 4 to 10. AMCA is a widely used fluorophore for multiple-color labeling due to its minimal fluorescence overlap with green- and longer wavelength-emitting fluorescent dyes.
Chemical Reactivity
Primary amines
Abs/Em Maxima
348/435 nm
Fluoresene quantum yield
0.91
Extinction Coefficient
17400
CF260
0.16
CF280
0.13
Spectrally Similar Dyes
Alexa Fluor® 350, AMCA, DyLight® 350