5-Aminofluorescein Chemical Properties

Melting point:

223 °C (dec.)(lit.)

Boiling point:

481.89°C (rough estimate)

Density 

1.3724 (rough estimate)

refractive index 

1.5180 (estimate)

storage temp. 

room temp

solubility 

DMF: 5 mg/ml; DMSO: 5 mg/ml

pka

9.34±0.20(Predicted)

form 

powder

color 

red-brown

Water Solubility 

Soluble in water (partly), methanol (10 mg/ml), and acetone.

BRN 

48395

Stability:

Light and Moisture Sensitive

InChIKey

GZAJOEGTZDUSKS-UHFFFAOYSA-N

CAS DataBase Reference

3326-34-9(CAS DataBase Reference)

EPA Substance Registry System

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-amino-3',6'-dihydroxy- (3326-34-9)

Safety Information

Hazard Codes 

Xn

Risk Statements 

22-36/37/38

Safety Statements 

26

WGK Germany 

3

F 

10

TSCA 

Yes

HS Code 

32049090

MSDS

• Language:English Provider:5-Amino-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich

5-Aminofluorescein Usage And Synthesis

Chemical Properties

Dark Red Solid

Uses

Glycosaminoglycuronans react with 5-aminofluorescein to yield fluorescent derivatives. 5-aminofluorescein is also used to prepare FITC Isomer I (F-011, F-020).

Uses

5-aminofluorescein is also used to prepare FITC Isomer I (CDX-F0011, CDX-F0020). It is a chemical used as molecular probe and important as fluorescent dye and for derivatives. Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens.

Definition

ChEBI: 5-aminofluorescein is a primary amino compound that is fluorescein carrying an amino substituent at C-5. Building block/intermediate for the synthesis of the fluorescent dye flourescein; also used to produce N-(fluorescein-5-yl)maleamic acid. It derives from a fluorescein (lactone form).

Preparation

The preparation of 5-aminofluorescein is carried out either by first saponification of 5-nitrofluorescein acetates with alcohol alkali, followed by reduction of the obtained 5-nitrofluorescein in a solution of alcohol alkali with hydrazine hydrate on a nickel catalyst to obtain 5-aminofluorescein , Or by boiling in an aqueous solution of sodium sulfide. The restoration of the nitro group and the saponification of acetates in the second case occurs in one stage.

General Description

Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins.

5-Aminofluorescein(3326-34-9)Related Product Information

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• AMINO ACIDS
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