Basic information Safety Supplier Related

Gambogenic acid

Basic information Safety Supplier Related

Gambogenic acid Basic information

Product Name:
Gambogenic acid
Synonyms:
  • Gambogenic acid
  • (2Z)-4-[(1R,3aS,5S,12aS)-9-[(2E)-3,7-Dimethyl-2,6-octadien-1-yl]-3a,4,5,7-tetrahydro-8,10-dihydroxy-3,3-dimethyl-11-(3-methyl-2-buten-1-yl)-7,13-dioxo-1,5-methano-1H,3H-furo[3,4-d]xanthen-1-yl]-2-methyl-2-butenoic acid
  • 2-Butenoic acid, 4-[(1R,3aS,5S,12aS)-9-[(2E)-3,7-dimethyl-2,6-octadien-1-yl]-3a,4,5,7-tetrahydro-8,10-dihydroxy-3,3-dimethyl-11-(3-methyl-2-buten-1-yl)-7,13-dioxo-1,5-methano-1H,3H-furo[3,4-d]xanthen-1-yl]-2-methyl-, (2Z)-
  • Gambogenic acid,Histone Methyltransferase,inhibit,Inhibitor
  • (Z)-4-((1R,3aS,5S,12aS)-9-((E)-3,7-Dimethylocta-2,6-dien-1-yl)-8,10-dihydroxy-2,2-dimethyl-11-(3-methylbut-2-en-1-yl)-4,7-dioxo-1,2,5,7-tetrahydro-1,5-methanofuro[2,3-d]xanthen-3a(4H)-yl)-2-methylbut-2-enoic acid
  • Gambogenic acid, 10 mM in DMSO
CAS:
173932-75-7
MF:
C38H46O8
MW:
630.78
Mol File:
173932-75-7.mol
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Gambogenic acid Chemical Properties

Boiling point:
818.6±65.0 °C(Predicted)
Density 
1.27±0.1 g/cm3(Predicted)
storage temp. 
4°C, protect from light
solubility 
DMSO : 250 mg/mL (396.34 mM; Need ultrasonic)
form 
Powder
pka
4.58±0.36(Predicted)
color 
Light yellow to yellow
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Gambogenic acid Usage And Synthesis

Chemical Properties

Brown-red crystalline powder, soluble in organic solvents such as methanol, ethanol, and DMSO, derived from Garcinia.

Uses

Gambogenic acid is an active ingredient that can be isolated from gamboge. Gambogenic acid acts as an effective inhibitor of EZH2, specifically and covalently binds to Cys668 within the EZH2-SET domain, and induces EZH2 ubiquitination. Gambogenic acid can be used for the research of cancer[1][2].

in vivo

Gambogenic acid (4-12 mg/kg, i.v., every 2 days, 14 days) inhibits tumor growth in SCLC mouse xenograft model[2].

Animal Model:SCLC mouse xenograft model[1]
Dosage:4-12 mg/kg
Administration:i.v., every 2 days, 14 days
Result:Increased the expression of pro-apoptotic proteins (cleaved caspase-3, -8 and -9, Bax, cleaved PARP and p53 proteins).
Decreased the anti-apoptotic proteins (Bcl-2).

IC 50

EZH2

References

[1] Wang X, et al. A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination. EMBO J. 2017 May 2;36(9):1243-1260. DOI:10.15252/embj.201694058
[2] Huang T, et al. Gambogenic acid inhibits the proliferation of small?cell lung cancer cells by arresting the cell cycle and inducing apoptosis. Oncol Rep. 2019 Mar;41(3):1700-1706. DOI:10.3892/or.2018.6950

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