Cy5.5 carboxylic acid Chemical Properties

solubility 

Soluble in DMSO, DMF, DCM

form 

Solid

color 

Brown to reddish brown

Appearance

Dark blue solid

ex/em

680/698 nm (PBS buffer)

ε(extinction coefficient)

198000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.20

InChIKey

MQFHWNVLHOOSOL-UHFFFAOYSA-N

SMILES

CC1(C(N(CCCCCC(=O)[O-])C2C=CC3=CC=CC=C3C1=2)=CC=CC=CC1=[N+](C2C=CC3=CC=CC=C3C=2C1(C)C)C)C

Cy5.5 carboxylic acid Usage And Synthesis

Description

Cy5.5 carboxylic acid is a free non-activated dye and can be used as a non-reactive fluorophore for experiment controls and instrument calibration. It is a non-sulfonated reagent with good solubility in organic solvents and limited aquous solubility.

Chemical Properties

Appearance: Dark blue solid
ex/em: 680/698 nm (PBS buffer)
Extinction coefficient (ε): 198000 L??mol??1??cm??1
Quantum yield (Φ): 0.20

Uses

CY5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye, is commonly used in bioimaging. CY5.5-COOH shows narrow absorption spectrum, and high sensitivity and stability[1].

References

[1] Sijoon Lee, et al. Toxicity and Biodistribution of Fragmented Polypropylene Microplastics in ICR Mice. Int J Mol Sci. 2023 May 9;24(10):8463. DOI:10.3390/ijms24108463

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