FOLIC ACID DIHYDRATE Chemical Properties

Melting point:

250°C

storage temp. 

Store at RT.

solubility 

Aqueous Acid (Slightly), Aqueous Base (Slightly), DMSO (Slightly)

pka

pK1:8.26 (25°C)

form 

Solid

color 

Yellow to Dark Yellow

Water Solubility 

Miscible with water.

Merck 

14,4221

BRN 

100781

CAS DataBase Reference

75708-92-8

Safety Information

WGK Germany 

3

RTECS 

LP5425000

TSCA 

Yes

MSDS

• Language:English Provider:SigmaAldrich

FOLIC ACID DIHYDRATE Usage And Synthesis

Chemical Properties

Yellow to yellow-orange crystalline powd

Uses

Vitamin B9. A B-complex vitamin necessary for the body to produce red blood cellsFolic acid dehydrate is used to form non-covalent complexes with polyethylenimine. It is used for the delivery of genes, which finds application in gene therapy. It is a B-complex vitamin, which is necessary for the body to produce red blood cells.

Purification Methods

If paper chromatography indicates impurities, then recrystallise it from hot H2O or from dilute acid [Walker et al. J Am Chem Soc 70 19 1948]. Impurities may be removed by repeated extraction with n-BuOH of a neutral aqueous solution of folic acid (by suspending in H2O and adding N NaOH dropwise till the solid dissolves, then adjusting the pH to ~7.0-7.5) followed by precipitation with acid, filtration, or better collected by centrifugation and recrystallised form hot H2O. [Blakley Biochem J 65 331 1975, Kalifa et al. Helv Chim Acta 6 1 2739 1978.] Chromatography on cellulose followed by filtration through charcoal has also been used to obtain pure acid. [Sakami & Knowles Science 129 274 1959.] UV: max 247 and 296nm ( 12,800 and 18,700) in H2O pH 1.0; 282 and 346nm ( 27.600 and 7,200) in H2O pH 7.0; 256, 284 and 366nm ( 24600, 24,500 and 86,00) in H2O pH 13 [Rabinowitz in The Enzymes (Boyer et al. Eds), 2 185 1960]. [Beilstein 26 III/IV 3944.]

FOLIC ACID DIHYDRATE(75708-92-8)Related Product Information

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• Folic acid