diSulfo-Cy5 amine Chemical Properties

storage temp. 

2-8°C, sealed storage, away from moisture and light

solubility 

Soluble in water, DMF, DMSO, alcohols

form 

Solid

color 

Brown to reddish brown

Appearance

Dark blue solid

ex/em

649/672 nm (PBS buffer)

ε(extinction coefficient)

271000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.28

InChIKey

YAAUASLQACUDFY-UHFFFAOYSA-N

SMILES

C([N+]1=C(C(C)(C)C2=CC(S([O-])(=O)=O)=CC=C12)C=CC=CC=C1N(C2=CC=C(S(O)(=O)=O)C=C2C1(C)C)C)CCCCC(=O)NCCCCCCN

diSulfo-Cy5 amine Usage And Synthesis

Description

Sulfo-Cy5 amine is a water-soluble dye that reacts with electrophilic substances and is very photostable. The addition of the amine group makes the compound reactive towards carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc.

Chemical Properties

Appearance: Dark blue solid
ex/em: 649/672 nm (PBS buffer)
Extinction coefficient (ε): 271000 L??mol??1??cm??1
Quantum yield (Φ): 0.28

Uses

Sulfo-Cy5 amine is a dye derivative of Cyanine 5 (Cy5) (HY-D0821) bearing an amine group. The sulfonate ion increases the water solubility of the compound, making it suitable for use in aqueous solutions. Cy5 is a near-infrared fluorescent dye commonly used in biolabeling and cell imaging. The amine functionality of Sulfo-Cy5 amine can react with carboxyl groups to form covalent bonds. Sulfo-Cy5 amine can bind to biomolecules such as proteins and antibodies to track their location and dynamic changes in biological samples.

Abs/Em Maxima

646/662 nm

Fluoresene quantum yield

0.28

Extinction Coefficient

271000

CF260

0.04

CF280

0.04

diSulfo-Cy5 amine(2183440-44-8)Related Product Information

• diSulfo-Cy5 maleimide
• 3H-IndoliuM, 2-[5-[1-[6-[(3-azidopropyl)aMino]-6-oxohexyl]-1,3-dihydro-3,3-diMethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-triMethyl-5-sulfo-, inner salt
• diSulfo-Cy5.5 alkyne
• diSulfo-Cy5 Hydrazide
• diSulfo-Cy5 DBCO(Methyl)
• diSulfo-Cy5 alkyne
• diSulfo-Cy3 amine
• Cy3 azide
• Cy5 Azide
• Cy5
• diSulfo Cyclohexane Cyanine7 carboxylic acid
• Sulfo-Cyanine5 carboxylic acid
• Cy7
• Sulfo Cy3 Carboxylic acids(methyl)
• Cy5.5 Acid (tri SO3)
• Cy5.5 Azide
• 1H-Benz[e]indolium, 2-[5-[3-[6-[(3-azidopropyl)amino]-6-oxohexyl]-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1,3-trimethyl-, chloride (1:1)
• diSulfo-Cyanine5 Me-tetrazine