5-FLUOROOROTIC ACID HYDRATE 98 Chemical Properties

Melting point:

278 °C (dec.)(lit.)

storage temp. 

Inert atmosphere,Store in freezer, under -20°C

solubility 

Aqueous Base (Heated), DMSO (Sparingly, Heated), Methanol (Sparingly), Water (Sl

form 

Solid

color 

Must be White

Water Solubility 

Soluble in water, dimethyl sulfoxide, 50mg in 1ml (1:1 ammonium hydroxide: water) with gentle heating.

Merck 

14,4176

BRN 

181933

Safety Information

Hazard Codes 

Xn

Risk Statements 

22-36/37/38

Safety Statements 

26-36

WGK Germany 

3

RTECS 

UV7800000

HazardClass 

IRRITANT

HS Code 

2933599590

5-FLUOROOROTIC ACID HYDRATE 98 Usage And Synthesis

Description

5-fluoro Orotic acid (5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs. Yeast with an active URA3 gene, which encodes orotine-5''-monophosphate decarboxylase, converts 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant strains of yeast that carry a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil.

Uses

5-Fluoroorotic Acid, Monohydrate (5-FOA) has become a valuable tool for research in the field of molecular genetics and has been employed in the selection of resistant strains of Saccharomyces cerevisiae that possess a mutant URA3 gene which renders them orotidine-5í-phosphate decarboxylation.

5-FLUOROOROTIC ACID HYDRATE 98(220141-70-8)Related Product Information

• Orotic acid
• 5-Fluoroorotic acid
• 5-FLUOROOROTIC ACID HYDRATE, 98