GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE Chemical Properties

storage temp. 

−20°C

solubility 

DMF: 20mg/mL; DMSO: 3mg/mL; PBS (pH 7.2): 3mg/mL

form 

A solid

InChIKey

UMNMBKCWESYUMR-LCJJWKFENA-N

SMILES

C1(C=CC([N+]([O-])=O)=CC=1)NC(=O)[C@@H](NC(=O)CN)CCCNC(=N)N.Cl.Cl |&1:12,r|

Safety Information

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE Usage And Synthesis

Description

H-Gly-Arg-pNA is a colorimetric substrate for thrombin. Thrombin preferentially binds to and cleaves the Gly-Arg (GR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of thrombin activity.

Uses

H-Gly-Arg-pNA hydrochloride (GR-pNA hydrochloride) is a colorimetric substrate for thrombin. H-Gly-Arg-pNA is preferentially bound by thrombin and cleaves the Gly-Arg (GR) peptide sequence, releasing p-nitroaniline (pNA) that can be detected colorimetrically at 405 nm. H-Gly-Arg-pNA hydrochloride is used as a fluorescent dye to measure thrombin activity[1].

References

[1] Hortin G L, et al. Macromolecular chromogenic substrates for measuring proteinase activity[J]. Clinical chemistry, 2001, 47(2): 215-222. PMID:11159769

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