Disodium 4-nitrophenylphosphate Chemical Properties

Melting point:

>300 °C(lit.)

Density 

1.751 at 20℃

vapor pressure 

15Pa at 20-50℃

storage temp. 

-20°C

solubility 

H₂O: 1 tablet/3 mL 25 mM, clear, light yellow

form 

tablets

color 

white to pale yellow

Water Solubility 

Miscible with water. Immiscible with ethanol and acetone.

BRN 

5324661

Stability:

Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents.

InChIKey

KAKKHKRHCKCAGH-UHFFFAOYSA-L

LogP

-2.16 at 22℃ and pH6.7

Surface tension

69.34mN/m at 1.051g/L and 20℃

CAS DataBase Reference

4264-83-9(CAS DataBase Reference)

EPA Substance Registry System

Phosphoric acid, mono(4-nitrophenyl) ester, disodium salt (4264-83-9)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36/38-36/37/38-48/22-41-38-21/22

Safety Statements 

26-36-24/25-23-36/37/39

WGK Germany 

3

F 

10-21

TSCA 

Yes

HS Code 

29199000

MSDS

• Language:English Provider:Disodium 4-nitrophenylphosphate
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

Disodium 4-nitrophenylphosphate Usage And Synthesis

Chemical Properties

White crystalline

Uses

PNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures.

General Description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm^-1 M^-1 at 311 nm in 0.4 M Na₂CO₃.

Biochem/physiol Actions

Primary TargetSubstrate for phosphatase

Purification Methods

Dissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.]

Disodium 4-nitrophenylphosphate(4264-83-9)Related Product Information

• Diphenyl chlorophosphate
• Triethyl phosphate
• Trisodium phosphate
• Pamidronate disodium salt
• Dibenzyl phosphate
• Sodium Phosphate, Dibasic
• Clodronate disodium
• Diphenylphosphoryl azide
• Tylosin phosphate
• PHOSPHATE STANDARD
• 4-Nitrobenzoic acid
• Ethylenediaminetetraacetic acid disodium salt
• Starch, hydrogen phosphate, 2-hydroxypropyl ether
• 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt
• Diphenyl phosphate
• AMPHISOL A
• Zinc phosphate
• Sodium Monofluorophosphate