SGX-523 Chemical Properties

Density 

1.485

storage temp. 

Store at -20°C

solubility 

≥17.95 mg/mL in DMSO

form 

solid

pka

3.94±0.10(Predicted)

color 

Light yellow to yellow

InChI

InChI=1S/C18H13N7S/c1-24-11-13(10-20-24)16-6-7-17-21-22-18(25(17)23-16)26-14-4-5-15-12(9-14)3-2-8-19-15/h2-11H,1H3

InChIKey

BCZUAADEACICHN-UHFFFAOYSA-N

SMILES

N1C2C(=CC(SC3N4C(=NN=3)C=CC(C3=CN(C)N=C3)=N4)=CC=2)C=CC=1

SGX-523 Usage And Synthesis

Uses

SGX523 is a novel, ATP-competitive kinase inhibitor.

Definition

ChEBI: A member of the class of triazolopyridazines that is 6-(1-methylpyrazol-4-yl)[1,2,4]triazolo[4,3-b]pyridazine-3-thiol in which the thiol hydrogen is replaced by a quinolin-6-yl group.

Biological Activity

sgx-523 is a novel, potent, atp-competitive, and highly-selective hepatocyte growth factor receptor (met) inhibitor with ic50 value of 4 nm [1].sgx523 inhibits met autophosphorylation in gastric cancer cell line gtl16 and human lung carcinoma cell line a549, with ic50 of 40 nm and 12 nm, respectively [1]. additionally, tumor regression was observed in gastic cancer cell line gtl16 and human gbm cell line u87mg derived mouse xenograft models that are treated with sgx-523 by oral gavage [1].sgx523 has been shown to inhibit the phosphorylateion of mek, mapk, akt in brain cancer cell lines including u87, u373, daoy, as well as glioma stem cells 1228. the inhibition of mek in brain cancer cells and stem cells led to cell proliferation, g1/s cell cycle progression, cell migration, and cell invasion [2].

Synthesis

Method b: 6-bromoquinoline (550 mg, 2.64 mmol) and diisopropylethylamine (1.13 mL, 6.46 mmol) were dissolved in N,N-dimethylacetamide (DMA, 4 mL) under nitrogen protection and degassed by nitrogen bubbling for 20 min. Subsequently, tris(dibenzylideneacetone)dipalladium (105 mg, 0.108 mmol, Strem catalyst), Xantphos (125 mg, 0.216 mmol), and 6-(1-methyl-1H-pyrazol-4-yl)-[1,2,4]triazolo[4,3-b]pyridazine-3-thiol (500 mg, 2.155 mmol ). The reaction mixture was stirred at 100 °C for 22 h until the solid was completely dissolved. After completion of the reaction, the mixture was cooled to room temperature and filtered through a short column of silica gel, using N,N-dimethylformamide (DMF) as eluent. The filtrate was poured directly into an ice/water mixture and allowed to stand for 15 minutes to precipitate the product. The precipitate was collected by filtration and washed with water. The resulting solid was ground with ether, filtered and dried under vacuum to give 770 mg of yellow crude product. The crude product was stirred in refluxing isopropanol for 1 h. The product was hot filtered, washed with isopropanol, and dried in a vacuum oven at 70 °C for 3 days to give a final 500 mg of 6-((6-(1-methyl-1H-pyrazol-4-yl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)sulfanyl)quinoline as a yellow solid with 8% impurities (64% yield).

in vivo

storage

Store at +4°C

References

[1] buchanan sg1, hendle j, lee ps, smith cr, bounaud py, jessen ka, tang cm, huser nh, felce jd, froning kj, peterman mc, aubol be, gessert sf,sauder jm, schwinn kd, russell m, rooney ia, adams j, leon bc, do th, blaney jm, sprengeler pa, thompson da, smyth l, pelletier la, atwell s, holme k,wasserman sr, emtage s, burley sk, reich sh. sgx523 is an exquisitely selective, atp-competitive inhibitor of the met receptor tyrosine kinase with antitumor activity in vivo. mol cancer ther. 2009 dec;8(12):3181-90.
[2] guessous f1, zhang y, dipierro c, marcinkiewicz l, sarkaria j, schiff d, buchanan s, abounader r.an orally bioavailable c-met kinase inhibitor potently inhibits brain tumor malignancy and growth. anticancer agents med chem. 2010 jan;10(1):28-35.

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