BIS-Tris
BIS-Tris Basic information
- Product Name:
- BIS-Tris
- Synonyms:
-
- 2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE
- 2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL
- 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol
- Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane
- Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade
- Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS
- Bis-Tris (2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol)
- BIS-TRIS BIOULTRA, >= 99.0% (NT)
- CAS:
- 6976-37-0
- MF:
- C8H19NO5
- MW:
- 209.24
- EINECS:
- 230-237-7
- Product Categories:
-
- Buffer
- API intermediates
- Mol File:
- 6976-37-0.mol
BIS-Tris Chemical Properties
- Melting point:
- 104 °C(lit.)
- Boiling point:
- 466.2±40.0 °C(Predicted)
- Density
- 1.346±0.06 g/cm3(Predicted)
- vapor pressure
- 0Pa at 25℃
- storage temp.
- Inert atmosphere,Room Temperature
- solubility
- H2O: 1 M at 20 °C, clear, colorless
- form
- Solution
- color
- Yellow to orange
- Odor
- Odorless
- PH Range
- 5.8 - 7.2
- PH
- 9.0-10.1 (20-25℃, 1M in water)
- pka
- 6.5(at 25℃)
- Water Solubility
- soluble
- Sensitive
- Air Sensitive
- λmax
- λ: 280 nm Amax: 0.15
- BRN
- 2205275
- InChIKey
- OWMVSZAMULFTJU-UHFFFAOYSA-N
- LogP
- -2.26 at 20℃
- CAS DataBase Reference
- 6976-37-0(CAS DataBase Reference)
- EPA Substance Registry System
- 1,3-Propanediol, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)- (6976-37-0)
Safety Information
- Hazard Codes
- Xi,Xn
- Risk Statements
- 36/37/38-21/22-20/21/22
- Safety Statements
- 26-36-36/37/39-22
- WGK Germany
- 3
- F
- 10-23
- TSCA
- Yes
- HS Code
- 29221980
MSDS
- Language:English Provider:2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
- Language:English Provider:ACROS
- Language:English Provider:SigmaAldrich
- Language:English Provider:ALFA
BIS-Tris Usage And Synthesis
Chemical Properties
White/clear crystalline powder
Uses
Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.
Uses
It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin.
Uses
Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.
Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.
Definition
ChEBI: Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris.
Flammability and Explosibility
Not classified
Purification Methods
Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.
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BIS-Tris(6976-37-0)Related Product Information
- 4,4'-Methylenedianiline
- 2-(METHYLSULFONYL)ETHANOL
- Hydroxyethyl starch
- Tricine
- Tris(hydroxymethyl)nitromethane
- Hydroxyethyl Cellulose
- Tris(hydroxymethyl)aminomethane
- Methyl 2-hydroxyethyl cellulose
- 2-(2-Aminoethylamino)ethanol
- BUFFER SOLUTION PH = 6,00 (20 °C) (POTASSIUM DIHYDROGEN PHOSPHATE/SODIUM HYDROXIDE)
- Sodium isethionate
- 2-(ISOPROPYLAMINO)ETHANOL
- tert-Butylamine
- Triethylamine
- Diethanolamine
- 2-Amino-1,3-propanediol
- BIS-TRIS-D19
- Hydroxymethyl