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BIS-Tris

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BIS-Tris Basic information

Product Name:
BIS-Tris
Synonyms:
  • 2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE
  • 2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL
  • 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol
  • Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane
  • Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade
  • Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS
  • Bis-Tris (2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol)
  • BIS-TRIS BIOULTRA, >= 99.0% (NT)
CAS:
6976-37-0
MF:
C8H19NO5
MW:
209.24
EINECS:
230-237-7
Product Categories:
  • Buffer
  • API intermediates
Mol File:
6976-37-0.mol
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BIS-Tris Chemical Properties

Melting point:
104 °C(lit.)
Boiling point:
466.2±40.0 °C(Predicted)
Density 
1.346±0.06 g/cm3(Predicted)
vapor pressure 
0Pa at 25℃
storage temp. 
Inert atmosphere,Room Temperature
solubility 
H2O: 1 M at 20 °C, clear, colorless
form 
Solution
color 
Yellow to orange
Odor
Odorless
PH Range
5.8 - 7.2
PH
9.0-10.1 (20-25℃, 1M in water)
pka
6.5(at 25℃)
Water Solubility 
soluble
Sensitive 
Air Sensitive
λmax
λ: 280 nm Amax: 0.15
BRN 
2205275
InChIKey
OWMVSZAMULFTJU-UHFFFAOYSA-N
LogP
-2.26 at 20℃
CAS DataBase Reference
6976-37-0(CAS DataBase Reference)
EPA Substance Registry System
1,3-Propanediol, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)- (6976-37-0)
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Safety Information

Hazard Codes 
Xi,Xn
Risk Statements 
36/37/38-21/22-20/21/22
Safety Statements 
26-36-36/37/39-22
WGK Germany 
3
10-23
TSCA 
Yes
HS Code 
29221980

MSDS

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BIS-Tris Usage And Synthesis

Chemical Properties

White/clear crystalline powder

Uses

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Uses

It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin.

Uses

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

Definition

ChEBI: Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris.

Flammability and Explosibility

Not classified

Purification Methods

Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.

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