BIS-Tris Chemical Properties

Melting point:

104 °C(lit.)

Boiling point:

466.2±40.0 °C(Predicted)

Density 

1.346±0.06 g/cm3(Predicted)

vapor pressure 

0Pa at 25℃

storage temp. 

Inert atmosphere,Room Temperature

solubility 

H₂O: 1 M at 20 °C, clear, colorless

form 

Solution

color 

Yellow to orange

PH Range

5.8 - 7.2

Odor

Odorless

PH

9.0-10.1 (20-25℃, 1M in water)

pka

6.5(at 25℃)

Water Solubility 

soluble

Sensitive 

Air Sensitive

λmax

λ: 280 nm Amax: 0.15

BRN 

2205275

InChIKey

OWMVSZAMULFTJU-UHFFFAOYSA-N

LogP

-2.26 at 20℃

CAS DataBase Reference

6976-37-0(CAS DataBase Reference)

EPA Substance Registry System

1,3-Propanediol, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)- (6976-37-0)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-21/22-20/21/22

Safety Statements 

26-36-36/37/39-22

WGK Germany 

3

F 

10-23

TSCA 

Yes

HS Code 

29221980

MSDS

• Language:English Provider:2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

BIS-Tris Usage And Synthesis

Chemical Properties

White/clear crystalline powder

Uses

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Uses

It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin.

Uses

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

Definition

ChEBI: Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris.

Flammability and Explosibility

Not classified

Purification Methods

Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.

BIS-Tris(6976-37-0)Related Product Information

• 4,4'-Methylenedianiline
• 2-(METHYLSULFONYL)ETHANOL
• Hydroxyethyl starch
• Tricine
• Tris(hydroxymethyl)nitromethane
• Hydroxyethyl Cellulose
• Tris(hydroxymethyl)aminomethane
• Methyl 2-hydroxyethyl cellulose
• 2-(2-Aminoethylamino)ethanol
• BUFFER SOLUTION PH = 6,00 (20 °C) (POTASSIUM DIHYDROGEN PHOSPHATE/SODIUM HYDROXIDE)
• Sodium isethionate
• 2-(ISOPROPYLAMINO)ETHANOL
• tert-Butylamine
• Triethylamine
• Diethanolamine
• 2-Amino-1,3-propanediol
• BIS-TRIS-D19
• Hydroxymethyl