PARP Chemical Properties

storage temp. 

−70°C

form 

buffered aqueous glycerol solution

biological source

rabbit

Safety Information

Hazard Codes 

B,T,N,Xn

Risk Statements 

20/22-24-37/38-41-51/53-43-36-20/21/22

Safety Statements 

23-24/25-45-36/37/39-26-61-36/37

WGK Germany 

1

MSDS

• Language:English Provider:SigmaAldrich

PARP Usage And Synthesis

Uses

PARP-1 is a nuclear enzyme that synthesizes ADP-ribose polymers from NAD⁺, specifically binds Zn²⁺ and DNA, and recognizes single-strand breaks in DNA. PARP1 has been used in a study to assess racial and tissue-specific cancer risk associated with polymorphism in the PARP1 gene. It has also been used in a study to investigate inhibitors of PARP-1 for potential cancer treatments.

General Description

Poly(ADP-ribose) polymerase 1 (PARP-1) is associated with the inflammation response during atherosclerosis.

Biochem/physiol Actions

PARP-1 is inactivated by cleavage into a 24kDA and 89kDA fragment by activated caspase-3 or caspase-7. This results in the decreased ability to repair DNA damage and an increase in apoptosis.

Description

PARP1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 354 amino acids (662-1014a.a.) and having a molecular mass of 39.6 kDa. PARP1 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli

Background

PARP1 takes part in the base excision repair pathway, by catalyzing the poly ADP-ribosyl of a restricted number of acceptor proteins involved in chromatin architecture and in DNA metabolism.. PARP1 mediates the poly ADP-ribosy of APLF and CHFR. PARP1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. PARP1 is a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly ADP-ribosyl. PARP1 takes part in the regulation of various significant cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. PARP1 is a site of mutation in Fanconi anemia, and is involved in the pathophysiology of type I diabetes.

PARP()Related Product Information

• 1-[[4-[(4-Fluoro-2-methyl-1H-indol-5-yl)oxy]-5-methylpyrrolo[2,1-f][1,2,4]triazin-6-yl]oxy]-2-propanol
• Ribose
• Gasdermin-A
• HumanKGF-2
• phosphodiesterase 4A, cAMP-specific
• Inorganic pyrophosphate
• Tyrosine-protein kinase Srms
• mTNFα
• Cys-Protein G
• EEK/FC CHIMERA