Coumarin 120 Chemical Properties

Melting point:

223-226 °C (lit.)

Boiling point:

306.47°C (rough estimate)

Density 

1.1999 (rough estimate)

refractive index 

1.5060 (estimate)

storage temp. 

2-8°C

solubility 

Soluble in acetone (10 mg/ml) dimethyl sulfoxide (10 mg/ml) and dimethylformamide.

form 

Crystalline Powder

pka

1.89 ± 0.20, most basic, temperature: 25 °C

color 

Beige to brown

Sensitive 

Light Sensitive

λmax

351 nm (MeOH); 342 nm (Buffer pH 7)

BRN 

142231

Major Application

For authentification; dyelasers; color display devices; inks;monitoring underlying corrosion on coated aluminum;photoinitiating systems; recording materials;semiconductor devices; dye-sensitized solar cells(DSCs); mesoporous silica fibers; SiO2 sonogelhybrid materials; textiles; waveguides;whitening agents,

Biological Applications

Identifyingbacteria; measuring urinary kallikrein levels;monitoring platinum(iv) reduction and platinum(ii)reactions in cancer cells; as a substrate for measuringadipinylamidases activity, aromatic amino aciddecarboxylases activity, histone deacetylasesactivity, hydrolases/esterases activity,β-lactamases activity, microbial nitroreductasesactivity, penicillin G acylases activity,proteases/proteinases/peptidases activity,glutamyltransferases activity, tryptophanasesactivity; as an effective antitubercular agent; use astemperature sensor,

InChIKey

GLNDAGDHSLMOKX-UHFFFAOYSA-N

CAS DataBase Reference

26093-31-2(CAS DataBase Reference)

NIST Chemistry Reference

Coumarin, 7-amino-4-methyl-(26093-31-2)

EPA Substance Registry System

2H-1-Benzopyran-2-one, 7-amino-4-methyl- (26093-31-2)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-20/21/22

Safety Statements 

26-36-37/39

WGK Germany 

3

Hazard Note 

Irritant

TSCA 

Yes

HS Code 

32049000

MSDS

• Language:English Provider:4-Methyl-7-aminocoumarin
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Coumarin 120 Usage And Synthesis

Chemical Properties

Coumarin 120 is Light yellow beige powder

Uses

Coumarin 120 is widely used fluorophore, well-known for preparing fluorogenic substrates for cystine aminopeptidase and other hydrolases. Used as reference compound in enzyme assays.

Uses

suitable for determining polygalacturonase activity

Uses

λ max 354 nm in EthanolSuitable as laser dye and used as a fluorescent labeling reagent for trace determination of enzymes.

Definition

ChEBI: Coumarin 120 is a member of 7-aminocoumarins. It has a role as a fluorochrome.

General Description

7-Amino-4-methylcoumarin (coumarin120) is an aminocoumarin laser dye that can emit laser in the range of 370-760 nm. It has three intermolecular hydrogen bonded structures with C-H···O and N-H····O.

Biochem/physiol Actions

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

storage

+4°C

Purification Methods

Dissolve it in 5% HCl, filter and basify with 2M ammonia. The precipitate is dried in a vacuum and recrystallised from dilute EtOH. It yields a blue solution and is light sensitive. [Sigler Synthesis 614 1980, Kanaoka et al. Chem Pharm Bull Jpn 30 1485 1982, Beilstein 18/11 V 445.]

Coumarin 120(26093-31-2)Related Product Information

• Lithium bis(trimethylsilyl)amide
• Betaine
• Dihydrocoumarin
• Methylparaben
• Acetonitrile
• Kresoxim-methyl
• 4-Methylcumarin
• Methanol
• N,N-Dimethyl-1,4-phenylenediamine
• Paraquat dichloride
• 6-Methylcoumarin
• Tris(hydroxymethyl)aminomethane
• DICUMAROL
• 4-Methylumbelliferone
• Glycine
• Methyl acetate
• Coumarin
• Methyl acrylate