CHES Chemical Properties

Melting point:

≥300 °C

Density 

1.2045 (rough estimate)

vapor pressure 

0Pa at 25℃

refractive index 

1.5364 (estimate)

storage temp. 

Keep in dark place,Inert atmosphere,Room temperature

solubility 

H₂O: 0.5 M at 20 °C, clear, colorless

form 

Solid

color 

White

PH

3.0-5.0 (25℃, 0.5M in H2O)

pka

9.3(at 25℃)

PH Range

8.6 - 10.0

Odor

Odorless

Water Solubility 

Soluble

Sensitive 

Hygroscopic

λmax

λ: 260 nm Amax: 0.08
λ: 280 nm Amax: 0.05

Merck 

14,2055

BRN 

2967601

Stability:

Stable. Incompatible with strong oxidizing agents.

InChIKey

MKWKNSIESPFAQN-UHFFFAOYSA-N

LogP

-2.2 at 20℃

CAS DataBase Reference

103-47-9(CAS DataBase Reference)

EPA Substance Registry System

Ethanesulfonic acid, 2-(cyclohexylamino)- (103-47-9)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36-20/22

Safety Statements 

26-28-24/25-16-60

WGK Germany 

3

F 

3

TSCA 

Yes

HS Code 

29213099

MSDS

• Language:English Provider:CHES
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

CHES Usage And Synthesis

Chemical Properties

White/clear cryst. powder

Uses

CHES is zwitterionic buffer useful in pH range 8.6 to 10.0.CHES is widely utilized as a buffer which is used for investigations on pH-dependent processes in enzymology. It has been shown to have an unusually high affinity for the iodoacetate binding site of liver alcohol dehydrogenase.

Uses

2-(Cyclohexylamino)ethanesulfonic acid (CHES) has been used in the preparation of dimethyl trisulfide- polysorbate 80 (DMTS-PS80) formulation.

Definition

ChEBI: N-cyclohexyl-2-aminoethanesulfonic acid is an alkanesulfonic acid that is cyclohexanamine in which one of the amino hydrogens is substituted by a 2-sulfoethyl group. It is used as a buffer (pH range of 8.6-10.0) for studying pH-dependent processes in enzymology. It has a role as a Good's buffer substance. It is an alkanesulfonic acid and a secondary aliphatic amine.

Application

CHES belongs to the class of organic compounds known as cyclohexylamines. These are organic compounds containing a cyclohexylamine moiety, which consist of a cyclohexane ring attached to an amine group. It is often used as biological buffer. It can bind to hemagglutinin (HA) emulating with sialic acid (SA) and receptor binding site (RBS)-targeting broadly neutralizing antibodies.

General Description

CHES (2-(N-cyclohexylamino)ethanesulphonic acid) is regarded as Good′s buffers for its self-buffering and biocompatible feature. It is used in protein stabilization and does not interact with metals.

Flammability and Explosibility

Not classified

CHES (103-47-9)Related Product Information

• Dodecylbenzenesulphonic acid
• Ambroxol
• Cyclohexylamine hydrochloride
• Linear alkyl benzenesulphonate
• Citric acid
• 4-Morpholineethanesulfonic acid
• Ethanesulfonic acid
• Sodium N-cyclohexylsulfamate
• Cyclohexylamine
• Ammonium sulfamate
• 4-Amino-benzenesulfonic acid monosodium salt
• Molasses
• Dicyclohexylamine
• Sulfamic acid
• Folic acid
• Aniline-2-sulfonic acid
• N,N-Dimethylcyclohexylamine
• Nickel bis(sulphamidate)