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Triphenylformazan

Basic information Safety Supplier Related

Triphenylformazan Basic information

Product Name:
Triphenylformazan
Synonyms:
  • N'-(Phenylamino)-N-phenyliminobenzenecarboximidamide
  • 1,3,5-Triphenyl-1,2,4,5-tetraaza-1,3-pentadiene
  • DTTC Formazan
  • Triphenylformazan Nitrogen
  • 1,3,5-Triphenylformazane
  • Triphenylformazane
  • Diazene, phenylphenyl(phenylhydrazono)methyl-
  • Phenyl(2-phenylhydrazono)(phenylazo)methane
CAS:
531-52-2
MF:
C19H16N4
MW:
300.36
EINECS:
208-509-1
Product Categories:
  • marker
  • Formazans
  • Tetrazolium Salts & Formazans
Mol File:
531-52-2.mol
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Triphenylformazan Chemical Properties

Melting point:
169-170 °C
Boiling point:
431.59°C (rough estimate)
Density 
1.1454 (rough estimate)
refractive index 
1.6140 (estimate)
storage temp. 
Store at 2-8
solubility 
dioxane: 50 mg/mL, clear
pka
11.56±0.10(Predicted)
form 
powder to crystaline
color 
Orange to Brown to Dark red
BRN 
753057
CAS DataBase Reference
531-52-2(CAS DataBase Reference)
NIST Chemistry Reference
Triphenylformazan(531-52-2)
EPA Substance Registry System
Diazene, phenyl[phenyl(phenylhydrazono)methyl]- (531-52-2)
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Safety Information

Hazard Codes 
Xn,N
Risk Statements 
22-50/53
Safety Statements 
60-61-24/25-22
RIDADR 
UN 3077 9/PG 3
WGK Germany 
3
8-10-23
HS Code 
3212900000

MSDS

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Triphenylformazan Usage And Synthesis

Chemical Properties

Dark brown crystalline powder

Uses

Germination and viability tests.

Uses

1,3,5-Triphenyltetrazolium formazan has been used:

  • for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
  • as a standard to study the optical density of formazan produced by TTC-treated milled seeds
  • to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry

General Description

1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.

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