N-[1-(2,3-DIOLEYLOXY)PROPYL]-N,N,N-TRIMETHYLAMMONIUM CHLORIDE Chemical Properties

Melting point:

35-38°C

storage temp. 

-20°C

solubility 

Chloroform (Slightly)

form 

lyophilized powder

color 

White to Pale Yellow

Stability:

Store in Freezer, Hygroscopic

InChIKey

KSXTUUUQYQYKCR-LQDDAWAPSA-M

SMILES

C(C[N+](C)(C)C)(COC(=O)CCCCCCC/C=C\CCCCCCCC)OC(=O)CCCCCCC/C=C\CCCCCCCC.[Cl-]

Safety Information

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

N-[1-(2,3-DIOLEYLOXY)PROPYL]-N,N,N-TRIMETHYLAMMONIUM CHLORIDE Usage And Synthesis

Description

DOTAP chloride (1,2-Dioleoyl-3-trimethylammonium-propane chloride) is a potent cationic lipid widely used as a liposomal transfection reagent. DOTAP is also a strong activator of the innate immune system in animal cells and could serve as a potential candidate for the study of elicitors of plant immunity.

Description

DOTAP, 1,2-Dioleoyl-3-trimethylammonium propane is a di-chain, or gemini, cationic surfactant. DOTAP has been used for in vitro and in vivo nucleic acid and protein delivery.

Chemical Properties

White Foam

Uses

Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of DNA. Facilitates fusion of the complex with the plasma membrane of tissue culture cells, resulting in both uptake and

Uses

1,2-dioleoyloxy-3-(trimethylammonium)propane (DOTAP) chloride has been used for the preparation of cationic liposome and liposome-DNA complex. It has also been used to assess tumor necrosis factor α (TNF- α) production.

Biochem/physiol Actions

18:1 TAP (DOTAP) is a cationic lipid structure, used for in vitro and in vivo nucleic acid and protein delivery. The lipid composition and cell type affects the transfection potential of DOTAP(1,2-dioleoyl-3-trimethylammonium-propane). 18:1 TAP (DOTAP) mediates gene knockdown technique in hematopoietic stem cells (HSCs).

N-[1-(2,3-DIOLEYLOXY)PROPYL]-N,N,N-TRIMETHYLAMMONIUM CHLORIDE(132172-61-3)Related Product Information

• Cholesterol
• PG?(egg?PC)
• Cholesteryl sodium sulfate
• Egg phospholipids
• Sodium hyaluronate
• DODMA
• DOTAP
• Ammonium chloride
• DOTAP MESYLATE, 98
• N-[1-(2,3-DIOLEYLOXY)PROPYL]-N,N,N-TRIMETHYLAMMONIUM CHLORIDE,N-[1-(2,3-DIOLEOYLOXY) PROPYL]-N,N,N-TRIMETHYLAMMONIUM CHLORIDE,DOTAP CHLORIDE SALT,DOTAP
• DOTAP
• DOTAP methyl sulfate,DOTAP,DOTAP METHOSULFATE
• DOTAP MESYLATE
• DOTAP METHYL SULPHATE SALT
• DOTAP
• DOTAP(OH) MESYLATE
• DOTAP acetate
• DOTAP(OH)2 MESYLATE