5-BROMO-4-CHLORO-3-INDOLYL B-D- Chemical Properties

Melting point:

228-230°C

alpha 

-87～-94°(20℃/D)(c=1,H2O-DMF)(calculated on the dehydrous basis)

Density 

1.4241 (rough estimate)

refractive index 

1.6110 (estimate)

storage temp. 

-20°C

solubility 

DMF: soluble

form 

tablet

color 

White to off-white or colorless

Stability:

Hygroscopic

InChIKey

PSBARFROSMJLJR-OXLZRBRISA-N

EPA Substance Registry System

.beta.-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1) (114162-64-0)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-37/39-24/25

WGK Germany 

3

F 

8-10-21

HS Code 

29349990

5-BROMO-4-CHLORO-3-INDOLYL B-D- Usage And Synthesis

Chemical Properties

White Solid

Uses

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining.

Uses

Suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.

General Description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by?Escherichia coli.

5-BROMO-4-CHLORO-3-INDOLYL B-D-(114162-64-0)Related Product Information

• 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
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