Creatininase amidohydrolase Chemical Properties

storage temp. 

2-8°C

form 

lyophilized powder

EPA Substance Registry System

Creatininase (9025-13-2)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-21

MSDS

• Language:English Provider:SigmaAldrich

Creatininase amidohydrolase Usage And Synthesis

Uses

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.

Biochem/physiol Actions

Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.

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