5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Basic information
- Product Name:
- 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
- Synonyms:
-
- 5-BROMO-4-CHLORO-3-INDOYL PHOSPHATE, P-TOLUIDINE SALT
- 5-BROMO-4-CHLORO-3-INDOXYL PHOSPHATE, P-TOLUIDINE SALT
- 5-BROMO-4-CHLORO-1H-INDOL-3-YL PHOSPHATE P-TOLUIDINE
- 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE
- 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT
- 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE 4-TOLUIDINE SALT
- 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE, COMPOUNDED WITH 4-METHYLBENZENAMINE
- 5-BROMO-4-CHLORO-3'-INDOLYPHOSPHATE P-TOLUIDINE SALT
- CAS:
- 6578-06-9
- MF:
- C8H6BrClNO4P.C7H9N
- MW:
- 433.63
- EINECS:
- 229-506-1
- Product Categories:
-
- substrate
- Indoles
- Simple Indoles
- Mol File:
- 6578-06-9.mol
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Chemical Properties
- Melting point:
- 194-195℃
- storage temp.
- -20°C
- solubility
- DMF: 20 mg/mL
- form
- tablets
- color
- White to Orange to Green
- InChIKey
- QEIFSLUFHRCVQL-UHFFFAOYSA-N
- CAS DataBase Reference
- 6578-06-9(CAS DataBase Reference)
- EPA Substance Registry System
- 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9)
Safety Information
- Hazard Codes
- Xi
- Risk Statements
- 36/37/38-40
- Safety Statements
- 26
- RIDADR
- 2811
- WGK Germany
- 3
- F
- 3-8-10
- HazardClass
- 6.1
- PackingGroup
- III
- HS Code
- 29339190
MSDS
- Language:English Provider:SigmaAldrich
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Usage And Synthesis
Chemical Properties
White to light brown powder
Uses
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
General Description
BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt(6578-06-9)Related Product Information
- AMPHISOL A
- BCDMH
- 4-Methylaniline hydrochloride
- Trisodium phosphate
- Calcium phosphate
- Chlorobromoisocyanurate
- Sodium Phosphate Monobasic Monohydrate
- p-Toluidine
- CHLORODIBROMOMETHANE
- Tributyl phosphate
- phosphate
- 8-CHLOROADENOSINE-3',5'-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT
- Bromine
- BCIP,BCIP 2 K,BCIP DIPOTASSIUM SALT
- 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALT
- X-PHOS
- BCIP/NBT
- toluidine