Basic information Safety Supplier Related

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Basic information Safety Supplier Related

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Basic information

Product Name:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Synonyms:
  • 5-BROMO-4-CHLORO-3-INDOYL PHOSPHATE, P-TOLUIDINE SALT
  • 5-BROMO-4-CHLORO-3-INDOXYL PHOSPHATE, P-TOLUIDINE SALT
  • 5-BROMO-4-CHLORO-1H-INDOL-3-YL PHOSPHATE P-TOLUIDINE
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT
  • 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE 4-TOLUIDINE SALT
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE, COMPOUNDED WITH 4-METHYLBENZENAMINE
  • 5-BROMO-4-CHLORO-3'-INDOLYPHOSPHATE P-TOLUIDINE SALT
CAS:
6578-06-9
MF:
C8H6BrClNO4P.C7H9N
MW:
433.63
EINECS:
229-506-1
Product Categories:
  • substrate
  • Indoles
  • Simple Indoles
Mol File:
6578-06-9.mol
More
Less

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Chemical Properties

Melting point:
194-195℃
storage temp. 
-20°C
solubility 
DMF: 20 mg/mL
form 
tablets
color 
White to Orange to Green
InChIKey
QEIFSLUFHRCVQL-UHFFFAOYSA-N
CAS DataBase Reference
6578-06-9(CAS DataBase Reference)
EPA Substance Registry System
1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9)
More
Less

Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38-40
Safety Statements 
26
RIDADR 
2811
WGK Germany 
3
3-8-10
HazardClass 
6.1
PackingGroup 
III
HS Code 
29339190

MSDS

More
Less

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Usage And Synthesis

Chemical Properties

White to light brown powder

Uses

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

General Description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine saltSupplier

Suzhou Rosen Biotechnology Co.,Ltd. Gold
Tel
0512-87888687 13626191410
Email
rosenchemical@163.com
Shanghai JONLN Reagent Co., Ltd. Gold
Tel
400-0066400 13621662912
Email
422131432@qq.com
Jiangsu Hi-Pure Technology New Materials Co. Ltd. Gold
Tel
0512-62764072; 18051857768
Email
sales@hi-pure.cn
Guangzhou Yaoguang Technology Co., Ltd. Gold
Tel
13020159086
Email
yangjue@ygfuture.com
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Email
jkinfo@jkchemical.com