DEXTRANASE Chemical Properties

storage temp. 

2-8°C

form 

solution

color 

brown

biological source

Penicillium sp.

Specific Activity

400-800units/mg protein

EPA Substance Registry System

Dextranase (9025-70-1)

Safety Information

WGK Germany 

3

F 

3-10

MSDS

• Language:English Provider:SigmaAldrich

DEXTRANASE Usage And Synthesis

Chemical Properties

Decomposes high molecular viscous glucans into low-viscosity isomaltose and isomaltotriose. Hydrolyzes 1,3-β- and 1,4-β-glycosidic bonds in β-D-glucan to oligosaccharides and glucose. Suitable pH value for action: 6.0-6.5 for those produced by bacteria; 4.0-4.5 for those produced by mold. generally stable at pH 6-9, such as at pH 8.0 and 35 ℃ for 3h, the activity is basically unchanged, to 45 ℃ about 20% lower activity, 50 ℃ 70% lower. When the presence of calcium ions in the aqueous solution is extremely stable, and heat resistance has increased, glycerol to help prevent the decline in activity, and to contain 40% to 60% of the most effective. Glycerin helps to prevent the activity from decreasing, and it is most effective when it contains 40% to 60%. The product is off-white amorphous powder or liquid, can be added with carrier and diluent. It is soluble in water and largely insoluble in ethanol, chloroform and ether.

Uses

Dextranase from Penicillium sp has been used:

• in the digestion of exopolysaccharides from P. aeruginosa biofilms and dextran polymers
• as a component of trypticase soy broth (TSYPS) for Streptococcus mutans culture
• in microgel degradation

Uses

Dextranase from Chaetomium erraticum has been used:

• in microcarrier dissolution to produce multicellular 3D spheroids for drug discovery
• in in vitro digestion of extracellular polysaccharide (EPS) from the bacterial isolates
• in a study to investigate the immobilization of dextranase
• to investigate the optimization of process conditions for enzymatic modification of alternan

Uses

Dextranase can be used in preparation of dextran for clinical use.

General Description

Dextranase from fungi belong to the glycoside hydrolase families (GH) 49. Dextranase from Penicillium sp. are stable at varying temperature and pH.

Biochem/physiol Actions

An endodextranase that hydrolyzes -(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production that increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

DEXTRANASE(9025-70-1)Related Product Information

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