N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid Chemical Properties

Melting point:

~225 °C (dec.)

Density 

1.260 (estimate)

refractive index 

1.5500 (estimate)

storage temp. 

Keep in dark place,Sealed in dry,Room Temperature

solubility 

H₂O: 1 M at 20 °C, clear, colorless

form 

Crystalline Powder

color 

White

PH Range

6.8 - 8.2

Odor

Odorless

PH

3.5-5.0 (25℃, 1M in H2O)

pka

7.5(at 25℃)

Water Solubility 

SOLUBLE

λmax

λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.045

Merck 

14,9176

BRN 

1957061

InChIKey

JOCBASBOOFNAJA-UHFFFAOYSA-N

CAS DataBase Reference

7365-44-8(CAS DataBase Reference)

EPA Substance Registry System

Ethanesulfonic acid, 2-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- (7365-44-8)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

22-24/25-36-26

WGK Germany 

1

TSCA 

Yes

HS Code 

29221980

MSDS

• Language:English Provider:2-[Tris(hydroxymethyl)methylamino]-1-ethanesulfonic acid
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid Usage And Synthesis

Chemical Properties

White/clear crystalline powder

Uses

A structural analog to Tris buffer.

Uses

N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid (TES) probably exists in solution as a zwitterion. Because the pKa is 7.4 (physiological pH) this buffer has considerable potential for a variety of biological applications. A buffer using TES free acid can be prepared by titrating the free acid (sc-206102) with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH. Alternatively, solutions of equimolar TES free acid and TES sodium (sc-264384) can be mixed to attain a buffer of the desired pH.  It has been shown to be toxic in vitro, but this toxicity may be due to its ability to form metal chelate complexes that are toxic for cells. TES is a commonly used biological buffering agent.

Definition

ChEBI: N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid is a Good's buffer substance, pKa = 7.5 at 20 ℃. It is a member of ethanolamines, an amino sulfonic acid and a TES. It is functionally related to a taurine. It is a tautomer of a N-tris(hydroxymethyl)methyl-2-ammonioethanesulfonate.

Purification Methods

Crystallise the acid from hot EtOH containing a little water.

N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid(7365-44-8)Related Product Information

• Tris-MOPS Buffer,10×
• Sodium Citrate buffer(0.1mol/L, pH4.5, sterile)
• CHAPS
• TRIS hydrochloride
• PBS Tablets
• Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
• Phosphate buffer containing Tween 20 (PBS-T tablets)
• DIPSO
• 3-[N,N-Bis(hydroxyethyl)amino]-2-hydroxypropanesulphonic acid sodium salt
• TAPSO
• 3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid sodium salt
• N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid sodium salt
• TAPS
• N-(Tris(hydroxymethyl)methyl)-2-aminoethanesulfonic acid sodium salt
• N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid
• Tris(hydroxymethyl)aminomethane
• N-TRIS(HYDROXYMETHYL)METHYL-2-AMINOETHANESULFONIC ACID-D15
• 2-([2-HYDROXY-1,1-BIS(HYDROXYMETHYL)ETHYL]AMINO)ETHANESULFONIC ACID HEMISODIUM SALT