Solvent Red 27 Chemical Properties

Melting point:

120 °C (dec.)(lit.)

Flash point:

103℃

storage temp. 

room temp

solubility 

chloroform/ethanol (1:1): soluble1mg/mL

form 

powder

Colour Index 

26125

color 

Red to dark greenish-brown

Water Solubility 

Soluble in ethanol:chloroform (1:1). Insoluble in water.

λmax

518 nm, 359 nm

BRN 

3491382

Biological Applications

Detecting early stages of atherosclerotic lesion formation; treating obesity,hyperlipidemia,fatty liver,leptin production disorders; dental impression material; measuring phagocytosis

CAS DataBase Reference

1320-06-5(CAS DataBase Reference)

EPA Substance Registry System

C.I. Solvent Red 27 (1320-06-5)

Safety Information

Hazard Codes 

F,Xi

Risk Statements 

36/37/38-67-36-11

Safety Statements 

22-24/25-26-16-7

RIDADR 

UN1219 - class 3 - PG 2 - Isopropanol, solution

WGK Germany 

3

TSCA 

Yes

HS Code 

32041900

MSDS

• Language:English Provider:1-(2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo)-2-naphthol
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

Solvent Red 27 Usage And Synthesis

Description

Solvent Red 27 is also called Oil Red O. As a commonly used antioxidant, it also acts as a colorant. Solvent Red 27 distinguishes fuel without antioxidants and reduces carbon deposits in the combustion chamber.

Physical properties

Solvent Red 27 is a dark green to dark brown crystalline powder.

Uses

Oil Red O is suitable as a lipid and lipoprotein stain on cellulose acetate. It is one of the diazo dyes used for staining of neutral triglycerides and lipids. It is used to analyze muscle fibers. It acts as an agent that stains lipids, muscle fibers and alveolar macrophages. Further, it is also used as marking dye. It is an active component of red colored smokes in pyrotechnics.

Uses

Oil Red O has been used for the staining of lipid droplets and lipid-containing vacuoles in cells.

Definition

ChEBI: Oil red O is a bis(azo) compound that is 2-naphthol substituted at position 1 by a {4-[(2,5-dimethylphenyl)diazenyl]-2,5-dimethylphenyl}diazenyl group. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of naphthols. It is functionally related to a 2-naphthol.

Preparation

2-Amino-2′,3,4′,5-tetramethylazobenzene diazotization, and Naphthalen-2-ol coupling.

General Description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Properties and Applications

blue light red. Soluble in ethanol for yellow red, soluble in acetone, soluble in benzene. In concentrated sulfuric acid to green light blue, blue purple precipitation after dilution.

Solvent Red 27(1320-06-5)Related Product Information

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