Basic information Safety Supplier Related
ChemicalBook >  Product Catalog >  Biochemical Engineering >  Amino Acids and Derivatives >  Natural amino acids and derivatives >  Biocytin

Biocytin

Basic information Safety Supplier Related

Biocytin Basic information

Product Name:
Biocytin
Synonyms:
  • NE-BIOTYNYL-L-LYSINE
  • N-EPSILON-BIOTIN-L-LYSINE
  • N-EPSILON-(+)-BIOTINYL-L-LYSINE
  • N-EPSILON-BIOTINYL-L-LYSINE
  • N-EPSILON-D-BIOTINYL-L-LYSINE
  • LYSINE(BIOTINYL)-OH
  • L-BIOCYTIN
  • EPSILON-BIOTINOYL-L-LYSINE
CAS:
576-19-2
MF:
C16H28N4O4S
MW:
372.48
Product Categories:
  • Amino Acids & Derivatives
  • Biotin Derivatives
Mol File:
576-19-2.mol
More
Less

Biocytin Chemical Properties

Melting point:
~245 °C (dec.) (lit.)
alpha 
D25 +53° (c = 1.05 in 0.1N NaOH)
Boiling point:
748.0±60.0 °C(Predicted)
Density 
1.234
storage temp. 
-20°C
pka
2.53±0.24(Predicted)
optical activity
[α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)
Merck 
13,1227
BRN 
97197
InChIKey
BAQMYDQNMFBZNA-MNXVOIDGSA-N
More
Less

Safety Information

WGK Germany 
3
HS Code 
29362900

MSDS

More
Less

Biocytin Usage And Synthesis

Chemical Properties

White Solid

Uses

Useful for the synthesis of Biocytin containing peptides to study the mechanism of reactions catalyzed by biotin - containing enzymes

Uses

Biocytin can be used as a versatile fluorescent marker for neuroanatomical investigations and for a biotinidase assay.

Definition

ChEBI: A monocarboxylic acid amide that results from the formal condensation of the carboxylic acid group of biotin with the N6-amino group of L-lysine.

Biological Activity

Versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

Purification Methods

Recrystallise biocytin rapidly from dilute MeOH or Me2CO. It can also be recrystallised from H2O by slow evaporation or by dissolving in the minimum volume of H2O and adding Me2CO until solid separates. It is freely soluble in H2O and AcOH but insoluble in Me2CO. [Wolf et al. J Am Chem Soc 74 2002 1952, 72 1048 1950.] It has been purified by chromatography on superfiltrol-Celite, Al2O3 and by countercurrent distribution and then recrystallised [IR: Peck et al. J Am Chem Soc 74 1991 1952]. The hydrochloride recrystallises from aqueous Me2CO/HCl and has m 227o (dec). [Beilstein 27 III/IV 7984.]

BiocytinSupplier

Tianjin heowns Biochemical Technology Co., Ltd. Gold
Tel
400 638 7771
Email
sales@heowns.com
Shenzhen Chim Biotech Co., Ltd. Gold
Tel
0755-36320761-
Email
sales@chimchem.com
Avantpbc Chemical Tech(Shanghai) Co,Ltd. Gold
Tel
17802107445;021-3120-8408
Wuxi maimo topp biotechnology co., LTD Gold
Tel
Email
lisa-li@mimotopes.cn
J & K SCIENTIFIC LTD.
Tel
010-82848833- ;010-82848833-
Email
jkinfo@jkchemical.com;market6@jkchemical.com