5(6)-Carboxyfluorescein Chemical Properties

Melting point:

275 °C

Boiling point:

464.9°C (rough estimate)

Density 

1.3418 (rough estimate)

refractive index 

1.6380 (estimate)

storage temp. 

Keep in dark place,Inert atmosphere,Room temperature

solubility 

DMSO (Sparingly), Methanol (Slightly, Heated)

form 

Solid

color 

Light Yellow to Dark Brown

Water Solubility 

slightly soluble

InChIKey

CPRVUBZYHZGASQ-UHFFFAOYSA-N

SMILES

C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1

EPA Substance Registry System

Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36-36/37/38-20/21/22

Safety Statements 

37/39-26-36-22

WGK Germany 

3

TSCA 

Yes

HS Code 

29322090

MSDS

• Language:English Provider:5(6)-Carboxyfluorescein
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5(6)-Carboxyfluorescein Usage And Synthesis

Description

5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.

Chemical Properties

Dark-Orange to Brown Solid

Uses

A popular probe of determination of intracellular pH.

Uses

5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.

Uses

As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent

Definition

ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein.

Solubility in organics

5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively.

5(6)-Carboxyfluorescein(72088-94-9)Related Product Information

• 2',7'-Dichlorofluorescein
• Xanthene
• ERYTHROSIN B
• Fluorescein
• EC 1.14.14.3
• Fluorescein isothiocyanate
• Fluorescein Sodium
• 6-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester
• 5-Carboxyfluorescein
• 6-Carboxyfluorescein
• Carboxy styrene-butadiene latex
• 5-CARBOXYFLUORESCEIN DIACETATE
• (+)-1-a-Pinene
• 5(6)-CARBOXYFLUORESCEIN-NHS
• FLUOS
• 5(6)-Carboxyfluorescein N-succinimidyl ester
• (1R)-(-)-Nopol
• (1S)-(-)-alpha-Pinene