Biotinyl tyramide Chemical Properties

Boiling point:

735.8±60.0 °C(Predicted)

Density 

1.227±0.06 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

Soluble in DMSO (up to 35 mg/ml) or in Ethanol (up to 20 mg/ml)

pka

10.01±0.15(Predicted)

form 

solid

color 

White

Appearance

white solid

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.

InChI

InChI=1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1

InChIKey

VZWXNOBHWODXCW-ZOBUZTSGSA-N

SMILES

C1(=O)N[C@]2([H])[C@H](CCCCC(NCCC3=CC=C(O)C=C3)=O)SC[C@]2([H])N1

Biotinyl tyramide Usage And Synthesis

Description

Biotinyl tyramide is a reagent that has been used for tyramide signal amplification (TSA) via catalyzed reporter deposition (CARD). In CARD, a reporter enzyme, such as horseradish peroxidase (HRP) conjugated to a secondary antibody, is bound to the target of interest and catalyzes the covalent deposition of biotinyl tyramide to the sample. The sample is then probed by a detector, such as streptavidin-HRP, allowing detection via chromogenic or fluorescent methods. Biotinyl tyramide has been used in immunohistochemistry, ELISA, Western blot, and in situ hybridization applications.

Uses

Biotinyl Tyramide is a Biotin (B389040) derivative, a growth factor present in minute amounts in every living cell. Plays an indispensable role in numerous naturally occurring carboxylation reactions. Occurs mainly bound to proteins or polypeptides. The richest sources are liver, kidney, pancreas, yeast, and milk. The biotin content of cancerous tumors is higher than that of normal tissue.

Solubility in water

Biotinyl tyramide is supplied as a solid. A stock solution may be made by dissolving the biotin tyramide in the solvent of choice, which should be purged with an inert gas. It is soluble in organic solvents such as DMS0 and dimethyl formamide. The solubility of biotinyl tyramide in these solvents is approximately 30 mg/ml.
This product is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, biotinyl tyramide should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. Biotinyl tyramide has a solubility of approximately 0.16 mg/ml in a 1:5 solution of DMSO: PBS(pH 7.2) using this method.

storage

Store at -20°C

References

[1] MARK N. BOBROW. Catalyzed reporter deposition, a novel method of signal amplification application to immunoassays[J]. Journal of immunological methods, 1989, 125 1: Pages 279-285. DOI:10.1016/0022-1759(89)90104-x
[2] MARK F EVANS  Kumarasen C  Holly A Aliesky. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens.[J]. BMC Clinical Pathology, 2003, 3 1: 2. DOI:10.1186/1472-6890-3-2
[3] Quantification of a-tubulin by sandwich ELISA with signal amplification through niotinyl-tyramide or immune-PCR
[4] OWEN J EINARSON  Dipankar S. Self-biotinylation of DNA G-quadruplexes via intrinsic peroxidase activity.[J]. Nucleic Acids Research, 2017, 45 17: 9813-9822. DOI:10.1093/nar/gkx765
[5] JIWON HWANG  Peter J E. Proximity-dependent biotin labelling in yeast using the engineered ascorbate peroxidase APEX2.[J]. Biochemical Journal, 2016, 473 16: 2463-2469. DOI:10.1042/bcj20160106

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