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BroMosporine

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BroMosporine Basic information

Product Name:
BroMosporine
Synonyms:
  • BroMosporine
  • ethyl 3-Methyl-6-(4-Methyl-3-(MethylsulfonaMido)phenyl)-[1,2,4]triazolo[4,3-b]pyridazin-8-ylcarbaMate
  • N-[6-(3-Methanesulfonamido-4-methylphenyl)-3-methyl-[1,2,4]triazolo[4,3-b]pyridazin-8-yl]carbamate
  • CS-921
  • Carbamic acid, N-[3-methyl-6-[4-methyl-3-[(methylsulfonyl)amino]phenyl]-1,2,4-triazolo[4,3-b]pyridazin-8-yl]-, ethyl ester
  • BroMosporine USP/EP/BP
  • 11,Bromosporine,inhibit,AML,colorectal cancer,CDK,antitumor,T cells,HCT116,Apoptosis,HT29,anticancer,Epigenetic Reader Domain,Inhibitor,Cyclin dependent kinase
  • KASUMI-1,HIV,AIDS,Human immunodeficiency virus,MV4
CAS:
1619994-69-2
MF:
C17H20N6O4S
MW:
404.44
Product Categories:
  • Inhibitors
  • API
Mol File:
1619994-69-2.mol
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BroMosporine Chemical Properties

Density 
1.46±0.1 g/cm3(Predicted)
storage temp. 
Sealed in dry,Store in freezer, under -20°C
solubility 
Soluble in DMSO (up to 25 mg/ml)
pka
6.21±0.46(Predicted)
form 
solid
color 
Off-white
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months.
InChI
1S/C17H20N6O4S/c1-5-27-17(24)18-15-9-14(21-23-11(3)19-20-16(15)23)12-7-6-10(2)13(8-12)22-28(4,25)26/h6-9,22H,5H2,1-4H3,(H,18,24)
InChIKey
UYBRROMMFMPJAN-UHFFFAOYSA-N
SMILES
CC(C(NS(C)(=O)=O)=C1)=CC=C1C2=NN3C(C(NC(OCC)=O)=C2)=NN=C3C
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Safety Information

WGK Germany 
WGK 3
Storage Class
11 - Combustible Solids
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BroMosporine Usage And Synthesis

Description

Bromosporine (1619994-69-2) is a potent pan-Bromodomain (BRD) inhibitor.1?Bromosporine was able to reactivate HIV-1 replication in different latency models.2

Uses

Bromosporine is a broad spectrum inhibitor for bromodomains.

in vivo

Bromosporine (100 mg/kg; i.p.; daily for 10 days) shows better antitumor activity than individual when co-treated with 5-Fluorouracil.html" class="link-product" target="_blank">5-FU (HY-90006)[1].

Animal Model:Female BALB/c nude mice (5-6 weeks; injected with 1?×?106?cells/100?μL of HT116?cells)[1]
Dosage:100 mg/kg
Administration:i.p.; daily for 10 days
Result:Exhibited better antitumor activity than individual Bromosporine or 5-FU (HY-90006) when co-treated with the two agent.

target

bromodomain

IC 50

HIV-1

storage

Store at +4°C

Background

Bromosporine is a potent broad-spectrum bromodomain inhibitor. Bromodomain and extra-terminal protein family members BRD2, BRD3, BRD4, and BRDT associate with acetylated lysines on histones and transcription factors to regulate their function, and have emerged as targets for cancer therapy. Treatment with Bromosporine induced the expression of latent HIV-1 in primary CD4+ T cells by inhibiting BET activity, leading to improved viral clearance in conjunction with antiretroviral therapy.

References

[1] SARAH PICAUD. Promiscuous targeting of bromodomains by bromosporine identifies BET proteins as master regulators of primary transcription response in leukemia[J]. Science Advances, 2016, 2 10. DOI:10.1126/sciadv.1600760
[2] HANYU PAN. The bromodomain and extraterminal domain inhibitor bromosporine synergistically reactivates latent HIV-1 in latently infected cells.[J]. Oncotarget, 2017: 94104-94116. DOI:10.18632/oncotarget.21585

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