6-Carboxytetramethylrhodamine Chemical Properties

Melting point:

NA

storage temp. 

Keep in dark place,Inert atmosphere,2-8°C

solubility 

DMSO: soluble

form 

A solid

color 

Light brown to black

Appearance

Purple solid

ex/em

550/576 nm (MeOH/PBS)

ε(extinction coefficient)

90000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.1

InChI

InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)20-11-14(24(28)29)5-8-17(20)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChIKey

COCMHKNAGZHBDZ-UHFFFAOYSA-N

SMILES

C(C1C=CC(C(=O)O)=CC=1C1=C2C=CC(N(C)C)=CC2=[O+]C2C=C(N(C)C)C=CC1=2)(=O)[O-]

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

8-10

HS Code 

3204 90 00

MSDS

• Language:English Provider:SigmaAldrich

6-Carboxytetramethylrhodamine Usage And Synthesis

Description

6-Carboxytetramethylrhodamine is a fluorescent dye that has commonly been used for the covalent labeling of oligonucleotides for DNA analysis. It displays excitation/emission maxima of 543/572 nm, respectively. 6-Carboxytetramethylrhodamine has been used in various DNA-protein binding studies, DNA FRET experiments, and as a standard reporter or quencher dye in RT-PCR.

Chemical Properties

Grey Solid

Uses

Free acid form of 6-carboxytetramethylrhodamine single isomer.

Uses

A long-wavelength dye

References

[1] MAKSIM V. KVACH. Practical Synthesis of Isomerically Pure 5- and 6-Carboxytetramethylrhodamines, Useful Dyes for DNA Probes[J]. Bioconjugate Chemistry Bioconjugate, 2009, 20 8: 1673-1682. DOI: 10.1021/bc900037b
[2] CHENGLING HAN  Lily Z  Tingting Chen. Terbium chelate as donor label in time-resolved fluorescence energy transfer study of DNA hybridization[J]. Chemical Physics Letters, 2010, 500 4: Pages 323-326. DOI: 10.1016/j.cplett.2010.10.028

Excitation / Emission Maximum

540 nm/568 nm

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