RESORUFIN SODIUM SALT Chemical Properties

Melting point:

>300 °C(lit.)

storage temp. 

room temp

solubility 

Methanol (Slightly), Water (Slightly)

form 

Solid

color 

Dark Purple to Black

Water Solubility 

water: 1mg/mL, opaque

BRN 

174850

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-37-36

WGK Germany 

3

RTECS 

SP7698000

MSDS

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RESORUFIN SODIUM SALT Usage And Synthesis

Chemical Properties

Dark Purple Solid

Uses

Resorufin has been used to determine the cytochrome P450 (CYP) 2A5 coumarin 7-hydroxylase (COH) activity changes over a broad range of lipopolysaccharide (LPS) doses. It has also been used as an internal standard for ethoxyresurufin-o-deethylase (EROD) analysis.

Uses

Resorufin is a pink fluorescent dye that is not fluorescent until activated by UV light. Resorufin is an anionic tricyclic dye.

General Description

Resorufin is a fluorescent indicator.

Purification Methods

The free acid is purified by dissolving it in conc H2SO4 which on dilution precipitates resorufin and is filtered off, washed well with hot H2O and dried. [Eichler J Prakt Chem 139 113 1934.] Detailed UV and IR studies were reported by Musso & Mattheis Chem Ber 90 1814 1957]. [pKa: Musso & Rathjen Chem Ber 92 751 1959, Beilstein 27 1V 2263.] The monoacetate [1152-14-3] M 252.2, m 2 2 3o is a fluorogenic substrate used for hydrolytic enzymes, and its fluorescence has Emax 593nm (Eex 500nm, in 0.1M phosphate pH 8.0 with lipase) [Kitson & Kitson Biochem J 322 701 1997]. [Beilstein 27 IV 2263.] The O7-methylresorufin (7-methoxy-3H-phenoxazin-3-one) [5735-89-3] M 227.2, m > 2 2 0o has fluorescence at Emax 585nm (Eexcit 571 nm in deacylase solution) and is used to differentiate isoenzymes of cytochrome P-450. It is insoluble in H2O and dilute alkali but is soluble in EtOH and CHCl3 to give an orange yellow colour. [Kehrmann Justus Liebigs Ann Chem 372 352 1910, Beilstein 27 II 108.]

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