β-Glycerophosphate disodium salt pentahydrate Chemical Properties

Melting point:

>300°C

storage temp. 

2-8°C

solubility 

H₂O: 0.1 g/mL, clear, colorless

form 

White powder

color 

Whiteto Off-White

Water Solubility 

Soluble in water.

Merck 

14,8622

BRN 

3744328

Stability:

Hygroscopic

InChI

InChI=1S/C3H9O6P.Na.H2O.H/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;1H2;

InChIKey

PEMUISUYOHQFQH-UHFFFAOYSA-L

SMILES

O(P(O)(O)=O)C(CO)CO.[NaH].O

Safety Information

WGK Germany 

3

RTECS 

UA0600000

F 

10-21

TSCA 

Yes

HS Code 

29199000

β-Glycerophosphate disodium salt pentahydrate Usage And Synthesis

Description

β-Glycerophosphate is a protein phosphatase inhibitor that acts as a phosphate group donor in matrix mineralization studies. It promotes bone matrix mineralization when delivered to osteoblasts by providing a source of phosphate ions. β-Glycerophosphate (10 mM) accelerates calcification of cultured vascular smooth muscle cells through an alkaline phosphatase-related mechanism.

Chemical Properties

Solid

Uses

β-Glycerol Phosphate was shown to have promoted mineralization in vitro by modulating bone cell metabolic activity and/or serving as a local source of inorganic phosphate ions.

Uses

Sodium β-glycerophosphate pentahydrate is used as a phosphatase inhibitor. It promotes bone matrix mineralization while delivering to osteoblasts by providing a source of phosphate ions. It is used in the development of hydrogels and scaffolds, which finds applications in tissue engineering and cell growth. It is used as an additive in isolation mediums by providing phosphate ions to isolate. It is utilized to promote mineralization in vitro by modulating bone cell metabolic activity.

General Description

A phosphate group donor in matrix mineralization studies. Accelerates calcification in vascular smooth muscle cells. Also acts as a protein phosphatase inhibitor.

Biochem/physiol Actions

Beta-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression

Purification Methods

Crystallise it from water. [Attwood et al. Biochem J 253 389 1988, Beilstein 1 IV 2766.]

References

[1]. chung ch, golub ee, forbes e, et al. mechanism of action of beta-glycerophosphate on bone cell mineralization. calcif tissue int. 1992 oct;51(4):305-11.
[2]. fujimoto h, mabuchi i. isolation of cleavage furrows from eggs of regular sea urchins and identification of furrow-specific proteins. j biochem. 1997 sep;122(3):518-24.
[3]. lecanda f, avioli lv, cheng sl. regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. j cell biochem. 1997 dec 1;67(3):386-96.
[4]. shioi a, nishizawa y, jono s, et al. beta-glycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells. arterioscler thromb vasc biol. 1995 nov;15(11):2003-9.

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