Cyanine5 tetrazine Chemical Properties

solubility 

Soluble in DMSO, DMF, DCM, low solubility in water

form 

Solid

color 

Dark purple to black

Appearance

dark blue powder

Cyanine5 tetrazine Usage And Synthesis

Description

Cy5 hydrazide is a cyanine dye containing hydrazide, which can be used to label aldehydes and ketones. This reagent allows to label various carbonyl-containing molecules such as antibodies and other glycoproteins after periodate oxidation, proteins after oxidative stress or deamination, or reducing saccarides.

Cyanine5 hydrazide replaces carbonyl-reactive Cy5?, Alexa Fluor 647, DyLight 649 dyes.

Uses

Cy 5 hydrazide (Cyanine5 hydrazide) is a fluorescent dye for aldehydes and ketones. Cy 5 hydrazide can be used for labeling proteins[1].

References

[1] Yoshimura T, et al. A novel procedure for the assessment of the antioxidant capacity of food components. Anal Biochem. 2016 Aug 15;507:7-12. DOI:10.1016/j.ab.2016.05.002

Abs/Em Maxima

646/662 nm

Fluoresene quantum yield

0.2

Extinction Coefficient

250000

CF260

0.03

CF280

0.04