EGS Crosslinker Chemical Properties

Boiling point:

603.4±65.0 °C(Predicted)

Density 

1.52±0.1 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

acetic acid: water (1:1): ≤50mg/mL

form 

powder

color 

White to off-white

BRN 

9167119

InChI

InChI=1S/C18H20N2O12/c21-11-1-2-12(22)19(11)31-17(27)7-5-15(25)29-9-10-30-16(26)6-8-18(28)32-20-13(23)3-4-14(20)24/h1-10H2

InChIKey

QLHLYJHNOCILIT-UHFFFAOYSA-N

SMILES

O(N1C(CCC1=O)=O)C(=O)CCC(=O)OCCOC(=O)CCC(=O)ON1C(CCC1=O)=O

Safety Information

WGK Germany 

3

F 

10

HS Code 

29280000

MSDS

• Language:English Provider:SigmaAldrich

EGS Crosslinker Usage And Synthesis

Description

EGS Crosslinker is a cleavable crosslinker which can be cleaved using hydroxyamine. EGS Crosslinkers contain two NHS ester groups which can react with primary amines.

Uses

Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is commonly used as a crosslinker in chemical reactions. It is a homobifunctional crosslinking reagent that is reactive with bromoacetate amines and sulfhydryls.

Biochem/physiol Actions

Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is a homobifunctional, cleavable cross-linking reagent. It is mainly coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). The linkage provides a 12-atom spacer.

Properties and Applications

EGS crosslinker, Ethylene glycolbis(succinimidyl succinate), is a water-insoluble homobifunctional crosslinker that must be dissolved in DMSO or DMF before addition to an aqueous reaction. EGS crosslinking takes place through amine-reactive NHS esters at both ends of a 16.1-angstrom spacer arm. The 12-atom spacer arm of the EGS crosslinking reagent is cleavable and the spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule and can be used to label intracellular proteins since the molecule is cell membrane permeable. EGS crosslinker is often used in ChIP assays to determine DNA-protein binding interactions. The directly water-soluble analog of EGS is Sulfo-EGS.

EGS Crosslinker(70539-42-3)Related Product Information

• 2-HYDROXYETHYL ACETATE
• Ethylenebis(oxyethylenenitrilo)tetraacetic acid
• 2-(Vinyloxy)ethanol
• Ethylene Glycol Bis(propionitrile) Ether
• DST
• 3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester)
• ETHYLENE GLYCOL DISUCCINATE BIS(SULFO-N-
• DISULFOSUCCINIMIDYL TARTRATE
• DISUCCINIMIDYL SUBERATE
• Ethylene glycol
• ETHYLENE GLYCOLBIS(SULPHOSUCCINIMIDYLSUCCINATE)
• EGS Crosslinker
• SULFO-EGS