Janus Green B Chemical Properties

Melting point:

160 °C (dec.)(lit.)

vapor pressure 

2.6 mm Hg ( 20 °C)

solubility 

H₂O: 1 M hot, clear, colorless

Colour Index 

11050

form 

Powder

color 

≤10(APHA)

Water Solubility 

Soluble in water (20 mg/ml at 20°C), ethanol, and slightly soluble in alcohol.

λmax

660nm, 395nm

Merck 

14,5255

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Antimalarialagents; diagnosis of diseases related to amyloid accumulation; diagnostic assays; detecting fungi; nucleic acids; sugars

InChIKey

XXACTDWGHQXLGW-UHFFFAOYSA-M

CAS DataBase Reference

2869-83-2(CAS DataBase Reference)

EPA Substance Registry System

Phenazinium, 3-(diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenyl-, chloride (2869-83-2)

Safety Information

Hazard Codes 

T,Xn

Risk Statements 

60-40

Safety Statements 

22-24/25-36

WGK Germany 

3

RTECS 

SG1633000

TSCA 

Yes

HS Code 

32041900

MSDS

• Language:English Provider:Union Green B
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

Janus Green B Usage And Synthesis

Chemical Properties

green powder

Uses

Janus Green B is a stain that interacts with DNA and has been used for histology studies including mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue. With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells.

Uses

As dye for cotton, wool; as biological stain; in electrodeposition of copper, Brown, Fellows, US 2882209 (1959 to Udylite Res. Corp.).

Definition

ChEBI: Janus Green B chloride is an organic chloride salt. It has a role as a dye. It contains a Janus Green B cation.

Safety Profile

Poison by intraperitoneal and intravenous routes. Moderately toxic by ingestion, skin contact, subcutaneous, and intramuscular routes. Human systemic effects by inhalation: nausea or vomiting. A skin and severe eye irritant. Flammable liquid when exposed to heat or flame; can react with oxidizing materials. To fight fire, use alcohol foam, CO2, dry chemical. When heated to decomposition it emits toxic fumes of NOx. See also AMINES.

Purification Methods

The dye dissolves in H2O to give a bluish violet solution which becomes colourless when made 10M in NaOH. It dissolves in EtOH to give a blue-violet colour, filter from insoluble material, then add dry Et2O whereby the dye separates out leaving a small amount of blue colour in solution. Filter off the solid and dry it in a vacuum. Store it in a dark bottle. [Colour Index Vol 4, 3rd edn, 4015 1971.]

Janus Green B(2869-83-2)Related Product Information

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