Deoxyribonucleic acid sodium salt Chemical Properties

Density 

1.00 g/mL at 20 °C

storage temp. 

-20°C

solubility 

50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow

form 

buffered aqueous solution

color 

Off-white

EPA Substance Registry System

Deoxyribonucleic acids, salmon sperm (68938-01-2)

Safety Information

Hazard Codes 

B

Safety Statements 

22-24/25

WGK Germany 

3

RTECS 

HG1933000

F 

3-10-21

TSCA 

Yes

HS Code 

29349990

MSDS

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Deoxyribonucleic acid sodium salt Usage And Synthesis

Chemical Properties

off-white to light tan powder

Uses

Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

General Description

Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon.

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