5-CFDA, AM Chemical Properties

Boiling point:

737.0±60.0 °C(Predicted)

Density 

1.51±0.1 g/cm3(Predicted)

form 

Solid

color 

White to off-white

Appearance

Solid Powder

5-CFDA, AM Usage And Synthesis

Description

5-carboxyfluorescein diacetate acetoxymethyl ester is a cell-permeant fluorogenic esterase substrate. It can be converted by nonspecific esterases of living cells from a membrane-permeable, nonpolar, nonfluorescent substance to a polar product CF, indicating the integrity of the plasma membrane.

Uses

5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].

References

[1] Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21. DOI:10.1016/j.mimet.2012.03.005

5-CFDA, AM(124412-00-6)Related Product Information

• 5-CARBOXYFLUORESCEIN DIACETATE, ACETOXYMETHYL ESTER (5-CFDA, AM)
• 5-CFDA, AM
• METHYL 4-(1-HYDROXYETHYL)BENZOATE
• BCECF-AM
• 4-(9-HYDROXY-9-XANTHENYL)BENZOIC ACID
• 4-(HYDROXY-PHENYL-METHYL)-BENZOIC ACID METHYL ESTER
• (S)-4-(1-HYDROXYETHYL) BENZOIC ACID METHYL ESTER
• (R)-4-(1-HYDROXYETHYL) BENZOIC ACID METHYL ESTER
• 4-Methylbenzhydrol
• METHYL 4-BENZYLBENZOATE
• 5-Carboxyfluorescein
• 4-METHOXY-4'-METHYLBENZHYDROL
• 5-CARBOXYFLUORESCEIN DIACETATE
• 4-(DIPHENYLHYDROXYMETHYL)BENZOIC ACID