Sulforhodamine B Chemical Properties

Density 

1.472[at 20℃]

storage temp. 

room temp

solubility 

methanol: 1 mg/mL

Colour Index 

45100

form 

powder

color 

Brown to Very Dark Purple

Water Solubility 

95.3g/L at 20℃

BRN 

3886008

InChI

InChI=1S/C27H30N2O7S2.Na.H/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);;

InChIKey

XCRMYPBRGZRYEU-UHFFFAOYSA-N

SMILES

S(C1C=C(S(O)(=O)=O)C=CC=1C1C2=CC=C(N(CC)CC)C=C2[O+]=C2C=C(N(CC)CC)C=CC=12)([O-])(=O)=O.[NaH]

LogP

-2.2 at 20℃

EPA Substance Registry System

C.I. Acid Red 52 (3520-42-1)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

22-24/25-26-36/37/39

WGK Germany 

2

RTECS 

BP6750000

F 

8

TSCA 

Yes

HS Code 

32129000

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

Sulforhodamine B Usage And Synthesis

Chemical Properties

This substance is a deep red powder. It is easily soluble in water, forming a bluish-red solution with yellow fluorescence. When sodium hydroxide solution is added, it also appears bluish-red. Additionally, it is soluble in ethanol. In concentrated sulfuric acid, it turns orange-yellow and red after dilution.

Uses

Sulforhodamine B sodium salt has been used:

• for relative total cell mass quantification
• to investigate the viscosity of coacervates
• as a molecular rotor for microviscosity determination in aerosol droplets

Uses

Sulforhodamine B Sodium Salt is a rhodamine dye widely used in nonpermanent tattoos. Studies show that Sulforhodamine B can be used as a potential new therapeutic agent for the treatment of the acute respiratory distress syndrome. Dyes and metabolites, Environmental Testing

Application

Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.

Preparation

4-Formylbenzene-1,3-disulfonic acid and 3-(Diethylamino)phenol condensation, the products with sulfuric acid dehydration, and ferric chloride oxidation, and finally into sodium salt.

Definition

ChEBI: Sulforhodamine B is an organic sodium salt having 4-[3,6-bis(diethylamino)-2,7-dimethylxanthenium-9-yl]benzene-1,3-disulfonate as the counterion.

General Description

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.

Flammability and Explosibility

Not classified

Biochem/physiol Actions

Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.

Properties and Applications

bright blue light pink. Dark red light brown powder. Soluble in water, in blue light red, with fluorescence, also soluble in alcohol. The strong sulfuric acid dyes is orange, diluted for red, with sodium hydroxide solution is blue light red. Mainly used for wool, silk and polyamide fiber dyeing, also can be used for wool and a variety of fabric dyeing, still can be in wool and silk fabric printing directly, also can used for leather dyeing.

Standard	Light Fastness	Soaping		Persperation Fastness	Oxygen bleaching		Fastness to seawater
		Fading	Stain		Fading	Stain	Fading	Stain
ISO	3	3-4	3	4	1	2	3	2
AATCC	2-3	3	3	3	1	2	4	2-3

Sulforhodamine B(3520-42-1)Related Product Information

• Sulforhodamine G
• Sulfocostulide A
• Sulfocostulide B
• DL-Sulforaphane
• 3-Dimethylaminophenol
• N,N-DIETHYL-P-TOLUIDINE
• Sodium p-toluenesulfinate
• SODIUM P-TOLUENESULFONATE
• 4-ETHYLTHIOPHENOL
• Sodium p-styrenesulfonate
• 3-Dimethylaminoanisole
• SODIUM 4-TOLUENESULFINATE HYDRATE
• m-Anisidine
• 4-Ethylbenzenesulfonic acid
• ACID BLUE 1
• METHALLYL PHENYL ETHER
• SULFORHODAMINE B, ACID FORM,SULFORHODAMINE B
• 3-METHOXY-N-METHYLANILINE