Cy5 Chemical Properties

storage temp. 

2-8°C

solubility 

Water, DMSO, DMF

form 

Solid

color 

Brown to green black

InChIKey

HPICMEGAGMPYID-UHFFFAOYSA-N

SMILES

C(N1C(C(C)(C)C2=CC(S(O)(=O)=O)=CC=C12)=CC=CC=CC1C(C2=CC(S([O-])(=O)=O)=CC=C2[N+]=1CC)(C)C)CCCCC(=O)O

Safety Information

HS Code 

29339900

Cy5 Usage And Synthesis

Description

Sulfo-Cy5 dye is one of the most popular far-red fluorescent dyes. It is often a reagent of choice for protein and peptide labeling. Sulfo-Cy5 dye is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. Sulfo-Cy5 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.The carboxylic acid of Sulfo Cy5 dye is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. Cy5 Acid is also often used for control experiments, and for calibration.

Uses

CY5 is a photoswitchable cyanine dye. The photoswitching mechanism was investigated using thiol agents and it was concluded that photoconversion into the dark state is pH and thiol dependent and the dark state product is a cyanine-thiol adduct.

Chemical Reactivity

Primary amine

Abs/Em Maxima

648/671 nm

Extinction Coefficient

250,000 cm-1M-1

Spectrally Similar Dyes

Alexa Fluor® 647, DyLight® 649,

Cy5(146368-11-8)Related Product Information

• 3H-IndoliuM, 2-[5-[1-[6-[(3-azidopropyl)aMino]-6-oxohexyl]-1,3-dihydro-3,3-diMethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-triMethyl-5-sulfo-, inner salt
• CY5 NHS ESTER
• Calcein tetraethyl ester
• CY3 NHS ESTER
• Cy7
• Sulfo Cyanine3 NHS ester
• diSulfo-Cy3 amine
• 1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-, chloride (1:1)
• diSulfo-Cy5 amine
• diSulfo-Cy5 alkyne
• Cyanine5 NHS ester
• Cy5
• Cy5.5
• Cy3 NHS Ester
• Cy3
• diSulfo-Cy5 maleimide
• diSulfo-Cy5.5 alkyne
• diSulfo-Cy5 Hydrazide