Z-LEU-LEU-GLU-AMC Chemical Properties

Boiling point:

967.8±65.0 °C(Predicted)

Density 

1.253±0.06 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

DMSO: soluble

form 

solid

pka

4.43±0.10(Predicted)

color 

Solid lyophilized powder

Sequence

{Z}-Leu-Leu-Glu-{AMC}

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

Z-LEU-LEU-GLU-AMC Usage And Synthesis

Description

Z-LLE-AMC is a fluorogenic substrate for the caspase-like post-glutamate peptide hydrolase of the 26S proteasome or 20S proteolytic core. Caspase-like activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm. Z-LLE-AMC is typically used in cell lysates after experimental treatment.

Uses

Z-Leu-Leu-Glu-7-amido-4-methylcoumarin has been used as a fluorogenic substrate for peptidyl glutamyl peptide hydrolase (PGPH) in rat soleus muscle, neonatal cardiomyocytes and rat brain samples.

Uses

A fluorogenic substrate for the peptidylglutamyl-peptide hydrolysing (caspase-like) activity of the proteasome which may be stimulated in the presence of Mg2+ ions and rapidly inactivated by N-acetylimidazole

General Description

Z-Leu-Leu-Glu-7-amido-4-methylcoumarin is a fluoropeptide. It acts as a substrate for caspase-like activity.

Biochem/physiol Actions

Fluorogenic substrate for the measurement of the peptidylglutamyl-peptide hydrolyzing activity of the 20S proteasome.

storage

+4°C

References

[1] MARIAN ORLOWSKI. Regulation of the peptidylglutamyl-peptide hydrolyzing activity of the pituitary multicatalytic proteinase complex[J]. Biochemistry Biochemistry, 1991, 30 24: 5999-6005. DOI: 10.1021/bi00238a025
[2] ELKE GEIER. A Giant Protease with Potential to Substitute for Some Functions of the Proteasome[J]. Science, 1999, 283 5404. DOI: 10.1126/science.283.5404.978
[3] MOHAMED-AMINE HAMOUDA. The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells.[J]. Oncotarget, 2014, 5 15: 6252-6266. DOI: 10.18632/oncotarget.2193
[4] LAURA TADLOCK. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest.[J]. American journal of physiology. Cell physiology, 2003, 285 2: C277-85. DOI: 10.1152/ajpcell.00550.2002

Z-LEU-LEU-GLU-AMC(348086-66-8)Related Product Information

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