Z-LEU-LEU-GLU-AMC Chemical Properties

Boiling point:

967.8±65.0 °C(Predicted)

Density 

1.253±0.06 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

DMSO: soluble

form 

solid

pka

4.43±0.10(Predicted)

color 

Solid lyophilized powder

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

Z-LEU-LEU-GLU-AMC Usage And Synthesis

Description

Z-LLE-AMC is a fluorogenic substrate for the caspase-like post-glutamate peptide hydrolase of the 26S proteasome or 20S proteolytic core. Caspase-like activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm. Z-LLE-AMC is typically used in cell lysates after experimental treatment.

Uses

Z-Leu-Leu-Glu-7-amido-4-methylcoumarin has been used as a fluorogenic substrate for peptidyl glutamyl peptide hydrolase (PGPH) in rat soleus muscle, neonatal cardiomyocytes and rat brain samples.

Uses

A fluorogenic substrate for the peptidylglutamyl-peptide hydrolysing (caspase-like) activity of the proteasome which may be stimulated in the presence of Mg2+ ions and rapidly inactivated by N-acetylimidazole

General Description

Z-Leu-Leu-Glu-7-amido-4-methylcoumarin is a fluoropeptide. It acts as a substrate for caspase-like activity.

Biochem/physiol Actions

Fluorogenic substrate for the measurement of the peptidylglutamyl-peptide hydrolyzing activity of the 20S proteasome.

storage

+4°C

Z-LEU-LEU-GLU-AMC(348086-66-8)Related Product Information

• Z-LEU-LEU-ARG-AMC
• Z-LEU-ARG-7-AMINO-4-METHYLCOUMARIN
• Z-LEU-LEU-OH
• Z-LLL-AMC
• BENZYLOXYCARBONYL-L-LEUCYL-L-ARGINYLGLYCYLGLYCINE 4-METHYLCOUMARYL-7-AMIDE
• H-GLU(AMC)-OH
• MG-115
• Z-LEU-LEU-GLU-AMC
• Z-LEU-LEU-GLU-7-AMINO-4-TRIFLUOROMETHYLCOUMARIN
• GLUTAMIC ACID(ANILIDE)-OH