CALCEIN-AM Chemical Properties

Melting point:

>250℃

Boiling point:

982.7±65.0 °C(Predicted)

Density 

1.49±0.1 g/cm3(Predicted)

refractive index 

n20/D 1.479

Flash point:

85 °C

storage temp. 

-20°C

solubility 

DMSO: soluble10mg/mL, clear, colorless to slightly yellow

form 

powder

pka

2.66±0.50(Predicted)

color 

Colorless

λmax

< 300 nm

Biological Applications

Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

RIDADR 

NA 1993 / PGIII

WGK Germany 

3

F 

10-21

MSDS

• Language:English Provider:SigmaAldrich

CALCEIN-AM Usage And Synthesis

Uses

Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions.

Uses

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.

Uses

A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule

Definition

ChEBI: Calcein am is an organic heteropentacyclic compound that is calcein in which all four carboxy group hydrogens have been substituted by (acetyloxy)methoxy groups and the hyrodgens of the two hydroxy groups have been substituted by acetyl groups. It is a a non-fluorescent probe cleaved to a fluorescent probe by non-specific intracellular esterases. It has a role as a fluorochrome. It is an acetate ester, an organic heteropentacyclic compound, a gamma-lactone, an oxaspiro compound, a member of 2-benzofurans and a xanthene dye. It is functionally related to a calcein.

General Description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Biochem/physiol Actions

Fluorescent cell permeable derivative of calcein.

storage

-20°C

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