Basic information Reactions Chemical Properties Description Carcinogenicity storage Safety Supplier Related
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Tetramethylbenzidine

Basic information Reactions Chemical Properties Description Carcinogenicity storage Safety Supplier Related

Tetramethylbenzidine Basic information

Product Name:
Tetramethylbenzidine
Synonyms:
  • 1’-biphenyl)-4,4’-diamine,3,3’,5,5’-tetramethyl-(
  • 1’-biphenyl)-4,4-diamine,3,3’,5,5’-tetramethyl-(
  • 1’-biphenyl]-4,4’-diamine,3,3’,5,5’-tetramethyl-[
  • TMB-E
  • TMB EASY
  • TMB/H
  • TMB-B
  • TMB BLUE ONE-STEP SUBSTRATE SYSTEM
CAS:
54827-17-7
MF:
C16H20N2
MW:
240.34
EINECS:
259-364-6
Product Categories:
  • Biochemistry
  • Enzyme
  • Substrates
  • Enzyme substrates
  • Alphabetical ListingSubstrates, Buffers&Blockers
  • Detection Substrates
  • ELISA SubstratesELISA Substrates
  • Liquid Substrate SystemsEnzyme Substrates
  • Peroxidase
  • Peroxidase Substrates
  • Substrates by Enzyme
  • Substrates for Peroxidase
  • ELISA
  • Benzopyrans
  • Biphenyl & Diphenyl ether
  • Benzoquinones, etc. (Charge Transfer Complexes)
  • Charge Transfer Complexes for Organic Metals
  • Functional Materials
Mol File:
54827-17-7.mol
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Tetramethylbenzidine Chemical Properties

Melting point:
168-171 °C(lit.)
Boiling point:
100 °C
Density 
1
refractive index 
1.5519 (estimate)
storage temp. 
2-8°C
solubility 
Slightly soluble. <0.1 g/100 mL at 20°C.
pka
4.49±0.10(Predicted)
form 
tablet
color 
Cream
Water Solubility 
Slightly soluble. <0.1 g/100 mL at 20 ºC
Sensitive 
Light Sensitive
BRN 
2808541
Stability:
Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
CAS DataBase Reference
54827-17-7(CAS DataBase Reference)
EPA Substance Registry System
3,3',5,5'-Tetramethylbenzidine (54827-17-7)
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Safety Information

Hazard Codes 
Xi,Xn
Risk Statements 
36/38-36/37/38-22-41
Safety Statements 
26-36-36/37-39
RIDADR 
UN 2796 8/PG 2
WGK Germany 
3
RTECS 
DV2300000
8-10
TSCA 
Yes
HazardClass 
6.1
HS Code 
29215990
Hazardous Substances Data
54827-17-7(Hazardous Substances Data)

MSDS

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Tetramethylbenzidine Usage And Synthesis

Reactions

TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase.

Oxidation of TMB

Shows the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethylbenzidine diimine

The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at the wavelengths of 370 and 650 nm.

Chemical Properties

Tetramethylbenzidine is sensitive to prolonged exposure to light. Neutralizes acids in exothermic reactions to form salts plus water.Tetramethylbenzidine may be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.

Description

Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. Tetramethylbenzidine is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.

Carcinogenicity

It is not known if Tetramethylbenzidine is carcinogenic and the evidence is contradictory: Tetramethylbenzidine is not mutagenic by the Ames test, and did not induce formation of tumors in a single-arm study of 24 rats. On that evidence, it has been used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.

storage

Keep as concentrated solution, aliquot and store at 4ºC. Do not freeze.

Chemical Properties

White or light yellow solid

Uses

3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content.

General Description

Pale yellow crystals or off-white powder.

Air & Water Reactions

Insoluble in water.

Reactivity Profile

Tetramethylbenzidine is sensitive to prolonged exposure to light . Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.

Fire Hazard

Flash point data for Tetramethylbenzidine are not available, however, Tetramethylbenzidine is probably combustible.

TetramethylbenzidineSupplier

Suzhou Haiheng Biological Pharmaceutical Co., Ltd. Gold
Tel
0512-65045967-
Email
sales@haihengpharm.com
Alfa Aesar Gold
Tel
400-610-6006; 021-67582000
Email
saleschina@alfa-asia.com
Nanjing Search Biotech Co., Ltd Gold
Tel
025-81682922-0;025-85860978-0
Email
sales@searchbio.com.cn;sales@searchbio.com.cn
Hunan Hui Bai Shi Biotechnology Co., Ltd. Gold
Tel
0731-85529965- ;0731-85526065- ;0731-85525605
Email
3002708854@qq.com;3002778010@qq.com;3002732518@qq.com
Taizhou Chemedir Biopharm-tech. Co., Ltd Gold
Tel
0523-86200218
Email
sale1_cmd@chemedir.com