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4-CHLORO-1-NAPHTHOL

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4-CHLORO-1-NAPHTHOL Basic information

Product Name:
4-CHLORO-1-NAPHTHOL
Synonyms:
  • 4-chloro-1-nahphthol
  • 4-chloro-1-naphthaleno
  • 4-chloro-1-Naphthalenol
  • 4-CHLORO-1-NAPHTHOL FOR DETECTION OF PEROXIDASE FOR MICROSCOPY
  • 4-Chloro-1-naphthol [for Biochemical Research]
  • 1-Chloro-4-naphthol
  • 1-Hydroxy-4-chloronaphthalene
  • NSC 44345
CAS:
604-44-4
MF:
C10H7ClO
MW:
178.62
EINECS:
210-068-5
Product Categories:
  • Enzyme Substrates
  • Peroxidase Substrates
  • Substrates by Enzyme
  • Alphabetical ListingWestern Blotting
  • PeroxidaseWestern Blotting
  • Tablets
  • BiochemicalsWestern Blotting
  • Molecular Biology
  • Fluorescent Enzyme Substrates for ElectrophoresisEnzyme Substrates
  • Peroxidase SubstratesAnalytical Reagents
  • Fluorescent Enzyme Substrates
  • Fluorescent Probes, Labels, Particles and Stains
  • Microscopy Reagents
  • Stains and Dyes
  • Naphthalene derivatives
  • Biochemistry
  • Enzyme
  • Substrates
  • Molecular Biology Reagents
  • Peroxidase
  • Fluorescent Enzyme Substrates for ElectrophoresisWestern Blotting
  • Alphabetical ListingFluorescent Probes, Labels, Particles and Stains
  • Detection Substrates
  • Powders
Mol File:
604-44-4.mol
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4-CHLORO-1-NAPHTHOL Chemical Properties

Melting point:
118-121 °C(lit.)
Boiling point:
254.41°C (rough estimate)
Density 
密度
refractive index 
1.4575 (estimate)
Flash point:
73 °C
storage temp. 
-20°C
solubility 
methanol: 50 mg/mL, clear, colorless to very faintly yellow
form 
tablets (~32 mg each)
pka
9.11±0.40(Predicted)
color 
Off-white to gray to light brown
BRN 
509750
CAS DataBase Reference
604-44-4(CAS DataBase Reference)
EPA Substance Registry System
1-Naphthalenol, 4-chloro- (604-44-4)
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Safety Information

Hazard Codes 
Xi,T
Risk Statements 
36/37/38-39/23/24/25-20/21/22
Safety Statements 
26-36-45-36/37
RIDADR 
UN 2810 6.1/PG 3
WGK Germany 
3
8-9-23
TSCA 
Yes
HS Code 
29081990

MSDS

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4-CHLORO-1-NAPHTHOL Usage And Synthesis

Chemical Properties

Off white to tan crystals

Uses

4-Chloro-1-naphthol has been used to visualize protein bands in western blotting. It has also been used in staining polyvinylidene fluoride (PVDF) membrane by Nakane method.

Uses

Suitable for visualizing protein-peroxidase conjugates in western blotting.

Synthesis Reference(s)

Tetrahedron, 38, p. 2347, 1982 DOI: 10.1016/0040-4020(82)87012-9

General Description

4-Chloro-1-naphthol is primarily used for the detection of proteins, it gives a characteristic purple precipitate and the reactions are easily controllable. It can be used as a substitute for benzidine compounds, which are considered to be carcinogenic.

Biochem/physiol Actions

4-Chloro-1-naphthol serves as a substrate for horseradish peroxidase.

Purification Methods

Crystallise the naphthol from EtOH or CHCl3. [Beilstein 6 H 611, 6 II 582, 6 III 2933, 6 IV 4233.]

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