Xylene Cyanole FF Chemical Properties

Melting point:

295°C

storage temp. 

room temp

solubility 

H2O: complete1mg/mL, clear, blue

Colour Index 

42135

form 

Solid

color 

Green

PH Range

2.9 (purple) - 4.6 (green)(Methyl Orange - Xylene Cyanol)

Water Solubility 

Soluble in water.

Stability:

Stable. Moisture-sensitive. Incompatible with strong oxidizing agents.

CAS DataBase Reference

2650-17-1(CAS DataBase Reference)

EPA Substance Registry System

1,3-Benzenedisulfonic acid, 4-[[4-(ethylamino)-3-methylphenyl][4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl]-, monosodium salt (2650-17-1)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-24/25

WGK Germany 

3

TSCA 

Yes

HS Code 

32041900

MSDS

• Language:English Provider:Sodium hydrogen 4-[[4-(ethylamino)-m-tolyl][4-(ethylimino)-3-methylcyclohexa-2,5-dien-1-ylidene]methyl]benzene-1,3-disulphonate

Xylene Cyanole FF Usage And Synthesis

Chemical Properties

dark green or blue-grey crystals

Uses

Suitable as a tracking dye for DNA sequencing.

Uses

Xylene Cyanol FF is a tracking dye commonly used for nucleic acids. The compound has been used in both electrophoresis polyacrylamide and agarose gels for various evaluations including DNA sequencing. The compound has also been used in a mobility shift analysis of ssDNA. In addition, two highly sensitive spectrophotometric assays have been developed for the detection of platinum (IV) (sc-250738, Platinum(IV) chloride) and Chromium (sc-214714) with the use of Xylene Cyanol FF. As an organic dye-inactivated Sendai virus vaccine, Xylene Cyanol FF displayed strong protection against the virus.

Preparation

4-Formylbenzene-1,3-disulfonic acid (1 Moore) and N-ethyl-2-methylbenzenamine (2 Moore) condensation, garnish with heavy chromate salt-oxalic acid oxidation its product.

General Description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Properties and Applications

bright blue. Soluble in water to blue.

Xylene Cyanole FF(2650-17-1)Related Product Information

• o-Xylene
• Sodium acetate
• Xylene
• Sodium bicarbonate
• Musk xylene
• Hydrogen
• m-Xylene
• Hydrogen peroxide
• XYLENE CYANOL,Xylene cyanol FF, pure,XYLENE CYANOL FF, CI 43535,XYLENE CYANOL FF,XYLENE CYANOL FF, CI 43535,Xylene cyanol FF, pure,XYLENE CYANOL FF
• Sodium p-styrenesulfonate
• HYDROGEN CYANIDE
• METHYL ORANGE-XYLENE CYANOL
• Acid blue 1
• 4-ETHYLBENZENESULFONIC ACID SODIUM SALT
• 4-STYRENESULFONIC ACID, SODIUM SALT HYDR ATE
• 4-VINYLBENZENESULFINIC ACID SODIUM SALT
• 1H,5H,11H,15H-XANTHENO[2,3,4-IJ:5,6,7-I'J']DIQUINOLIZIN-18-IUM, 9-(2,4-DISULFOPHENYL)-2,3,6,7,12,13,16,17-OCTAHYDRO-, INNER SALT, SODIUM SALT
• Xylene Cyanole FF