5-Nitrofluorescein Chemical Properties

solubility 

Acetone, Methanol

form 

Solid

color 

Orange Cyrstalline

Stability:

Light and Moisture Sensitive

InChI

InChI=1S/C20H11NO7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)20(14)16-7-10(21(25)26)1-4-13(16)19(24)28-20/h1-9,22-23H

InChIKey

GYWGQAIDMYGOLU-UHFFFAOYSA-N

SMILES

C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=CC([N+]([O-])=O)=C1)C(=O)O2

EPA Substance Registry System

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-6-nitro- (27402-68-2)

Safety Information

HS Code 

29329990

5-Nitrofluorescein Usage And Synthesis

Chemical Properties

Orange Cyrstalline Solid

Uses

Fluorescent labeling reagent for proteins, 6-Nitrofluorescein can be used in the fluorescent antibody technique for rapid identification of pathogens.

Preparation

5-Nitrofluorescein (3′,6′-dihydroxy-6-nitrospiro[2-benzofuran-3,9′-xanthene]-1-one) is a key intermediate in the synthesis of various fluorescent compounds such as 5-aminofluorescein (5-amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one), fluorescein 5-isothiocyanate (FITC; 3′,6′-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one), and fluorescein lisicol (previously known as cholyl-lysylfluorescein or CLF.
Process for the preparation of 5-/6-nitrofluorescein(US20120220787A1)

5-Nitrofluorescein(27402-68-2)Related Product Information

• 5-Nitro-1,3-dihydroisobenzofuran
• N-Methyl-(2-thienylmethyl)amine
• ISOPROPYL 4-AMINOBENZOATE
• 2-Methyl-4-nitrobenzoic acid
• 4-Amino-2-methylbenzoic acid
• 5-Nitrofluorescein
• Fluorescein
• 5-Nitrofluorescein diacetate
• 5-NITROFLUORESCEIN,NITROFLUORESCEIN, ISOMER 1
• 4-Nitrofluorescein
• EOSIN B
• 6-Nitrofluorescein Diacetate
• 4-Methyl-3-(1-methylethyl)benzenamine
• 5-Aminophthalide
• Ethyl p-nitrobenzoate
• 3-(1-HYDROXYETHYL)ANILINE
• 6-Aminofluorescein
• METHYL M-NITROPHENYL CARBINOL