Cy3 methyltetrazine Chemical Properties

solubility 

Soluble in DCM, DMSO, DMF

Appearance

dark red solid

Cy3 methyltetrazine Usage And Synthesis

Description

Cy3 methyltetrazine is a fluorescent compound with a methyltetrazine moiety. Methyltetrazines possess optimal stability at physiological pH and is highly reactive towards cyclooctenes. This fluorophore is bright and very well visible with naked eye.

Uses

Cy3 methyltetrazine (TZ-Cy3) is a click chemistry reagent with methyltetrazine building blocks that is highly reactive towards cyclooctene. Cy3 methyltetrazine is also a tetrazine-modified fluorescent probe that can be used to analyze protein phosphorylation in solution and living cells[1].

References

[1] Li Y, et al. Analysis of protein phosphorylation in solution and in cells by using an ATP analogue in combination with fluorescence techniques. Analyst. 2021 Jul 21;146(14):4506-4514. DOI:10.1039/d1an00742d

Abs/Em Maxima

555/570 nm

Fluoresene quantum yield

0.31

Extinction Coefficient

150000

CF260

0.04

CF280

0.09