3,3'-Diaminobenzidine tetrahydrochloride hydrate Chemical Properties

Melting point:

300°C

storage temp. 

room temp

solubility 

H2O: soluble50mg/mL

form 

Powder

color 

White to Amber to Dark purple

Water Solubility 

Soluble at 5%(w/v) in water

Safety Information

Hazard Codes 

Xn,T

Risk Statements 

45-68-36-22

Safety Statements 

22-24/25-45-53-26

WGK Germany 

3

RTECS 

DV8753000

TSCA 

Yes

HS Code 

29215900

3,3'-Diaminobenzidine tetrahydrochloride hydrate Usage And Synthesis

Chemical Properties

Grey to brownish-purple crystalline powder

Uses

3,3'-diaminobenzidine-tetrahydrochloride-dihydrate(DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.

Uses

3,3′-Diaminobenzidine tetrahydrochloride hydrate can be used in HRP-based assays suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).

General Description

3,3′-Diaminobenzidine tetrahydrochloride hydrate, also, known as DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.

Biochem/physiol Actions

In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.

3,3'-Diaminobenzidine tetrahydrochloride hydrate(868272-85-9)Related Product Information

• 3,3',4,4'-Biphenyltetramine tetrahydrochloride
• Xylene
• N,N,N',N'-Tetramethylethylenediamine
• Diphenylamine
• 2,7-Diaminofluorene
• 3,3'-Diaminobenzidine
• 3,3'-Diaminobenzidine tetrahydrochloride dihydrate
• DAB TRIS TABLET
• 3,4-DIAMINOBIPHENYL DIHYDROCHLORIDE
• 3,3'-Diaminobenzidine tetrahydrochloride hydrate