Hot Start PCR Master Mix (Antibody modified,2×mix) Usage And Synthesis

characteristic

Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step.

Productivity

Better reaction outcomes:Higher yield of target amplicons from low template amounts;Increased reaction specificity due to reliable hot-start technology;Wider range of amplicon lengths - routine amplification of genomic DNA fragments up to 6kb.Enhanced convenience:Room temperature reaction set-up;Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer;Simplified workflow and minimized pipetting steps with 2X master mix format.

Description

Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq? Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. A version of this master mix is also available with pre-added density reagent and electrophoresis tracking dyes.

Application

Routine PCR,Colony PCR,Genotyping,RT-PCR,Generation of PCR products for TA cloning