7-AMINOACTINOMYCIN D Chemical Properties

Melting point:

252～253℃

Boiling point:

1400.8±65.0 °C(Predicted)

Density 

1.44

Flash point:

801℃

storage temp. 

-20°C

solubility 

chloroform: 1 mg/mL, clear, red

form 

powder

pka

8.90±0.70(Predicted)

color 

red to dark purple

Appearance

purple powder

BRN 

5915844

Stability:

Stable for 1 year from date of purchase as supplied. Solutions should be made fresh daily.

Safety Information

Hazard Codes 

T+,T

Risk Statements 

26/27/28-36/37/38

Safety Statements 

26-28-36/37-45

RIDADR 

UN 3462 6.1/PG 2

WGK Germany 

3

RTECS 

AU1579000

F 

8-10-21

HazardClass 

6.1(a)

PackingGroup 

II

HS Code 

29419090

MSDS

• Language:English Provider:SigmaAldrich

7-AMINOACTINOMYCIN D Usage And Synthesis

Description

7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that is commonly used for the detection or exclusion of non-viable cells in flow cytometric analysis, as it is generally excluded by live cells. It displays excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm. As 7-AAD is detected in the far red range of the spectrum, it exhibits minimal spectral overlap with commonly used probes, therefore it can be used in conjunction with probes such as FITC. 7-AAD has been used to evaluate apoptosis and cell-mediated cytotoxicity and to stain DNA in cells that have been fixed and permeabilized by a variety of methods.

Chemical Properties

Purple powder

Uses

Fluorescent DNA stain. A featured product for Apoptosis research.

Uses

7-Aminoactinomycin D is a fluorescent DNA dye for apoptosis and flow cytomtery studies.

Definition

ChEBI: 7-aminoactinomycin D is a chromopeptide. It has a role as a fluorochrome.

General Description

Free form:
Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nm
Emission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nm
DNA Complex:
Excitation: 543 nm; 555 nm
Emission: 655 nm; 665 nm

Biochem/physiol Actions

Primary Targetleukemias and sarcomas

storage

Store at -20°C

References

[1] TANJA TELIEPS. Cellular-FLIP, Raji isoform (c-FLIPR) modulates cell death induction upon T-cell activation and infection[J]. European Journal of Immunology, 2013, 43 6: 1499-1510. DOI:10.1002/eji.201242819
[2] INGRID SCHMID  Beth D J  Christel Uittenbogaart. Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D[J]. Nature Protocols, 2007, 2 1: 187-190. DOI:10.1038/nprot.2006.458
[3] K TOBA. Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY.[J]. European Journal of Haematology, 2000, 64 1: 10-21. DOI:10.1034/j.1600-0609.2000.09005.x
[4] HERVÉ LECOEUR. Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods[J]. Journal of immunological methods, 1997, 209 2: Pages 111-123. DOI:10.1016/s0022-1759(97)00138-5
[5] X SU. Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect.[J]. Journal of immunology, 1996, 156 11: 4198-4208.

Abs/Em Maxima

527 (complex)/640 (complex) nm

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