2-IMINOBIOTIN HYDROBROMIDE Chemical Properties

Boiling point:

477.3±55.0 °C(Predicted)

Density 

1.60

storage temp. 

2-8°C

solubility 

1 M HCl: 50 mg/mL, clear, colorless

pka

4.73±0.10(Predicted)

form 

White to off-white powder.

BRN 

18952

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-21

MSDS

• Language:English Provider:SigmaAldrich

2-IMINOBIOTIN HYDROBROMIDE Usage And Synthesis

Uses

2-Iminobiotin has been used as a competitive inhibitor to bind to biotic binding sites on streptavidin in order to restore fluorescence signal.

General Description

2-Iminobiotin is a cyclic guanidino analog of biotin that has high affinity for avidin at high pH (>9) and low affinity at low pH (<6). The proteins that are iminobiotinylated are selectively contained within the avidin column at pH 9 to 11 and may be eluted at pH 4 or by the addition of biotin. This technique may be very useful in the study of cell surface proteins by labeling and selectively recovering the components that are periodate-sensitive from intact human erythrocyte surface. 2-Iminobiotin reversibly inhibits NOS (nitric oxide synthases) via its guanidino group. It inhibits NO biosynthesis and may be important to understand the binding-site interactions for this important class of enzymes. NOS oxidizes the guanidino-nitrogen of L-arginine to produce nitric oxide and L-citrulline.

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