iodoHoechst 33258 Chemical Properties

Boiling point:

737.0±70.0 °C(Predicted)

Density 

1.587±0.06 g/cm3(Predicted)

solubility 

25℃: DMSO or water Protect from light

form 

Powder

pka

10.69±0.10(Predicted)

color 

Light yellow to yellow

Appearance

Solid Powder

iodoHoechst 33258 Usage And Synthesis

Description

Para-iodoHoechst 33258, an iodinated DNA-binding bibenzimidazole, is a cell dye for DNA quantitation which sensitizes DNA and cells to UVA.

Uses

para-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

References

[1] a b c "Hoechst Stains". Invitrogren (Molecular Probes).
[2] Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68. DOI:10.1016/0167-4781(88)90079-6